Oligonucleotide Error Filtering for Gene Synthesis

描述（由申请人提供）：Genetic Assemblies Inc.提议开发一种错误过滤器，用于消除在合成低核苷酸和更长的DNA片段过程中引入的错误。该过滤器是自动基因合成器（AGS）的关键使能技术，该项目目前正在Genetic Assemblies Inc. （GA）开发中。AGS的目标是在不到24小时内产生长度达10,000 bp的用户定义DNA片段，并且基本上没有引入错误。该误差滤波器将与现有的无掩模阵列合成器（MAS）的大规模并行、光导DNA合成技术和由GA创始人在威斯康星大学麦迪逊分校开发的光导寡核苷酸释放化学技术集成，并授权给GA。该项目的具体目标包括：将错误结合蛋白附着在固体载体上，确定错误过滤器的结合能力和最佳结合条件，确定最佳错误过滤方法。生产无差错长DNA片段的能力将彻底改变分子生物学领域。它将允许生产定制设计的DNA，用于蛋白质工程、DNA疫苗、基因治疗和其他与改善人类健康有关的研究。这种能力将大大减少科学界对目前用于基因研究的大量克隆DNA片段的依赖，因为几乎任何已知序列的DNA都可以按需合成。这项技术将大大加快基因研究的步伐，并导致新的药物，治疗遗传病和先进的基因技术的发展。

DESCRIPTION (provided by applicant): Genetic Assemblies Inc. proposes the development of an error filter for the elimination of errors introduced during the synthesis of oligonucleotides and longer DNA fragments. This filter is a critical enabling technology for an Automatic Gene Synthesizer (AGS), a project now under development at Genetic Assemblies Inc. (GA). The goal of the AGS is to produce user-defined DNA fragments of up to 10,000 bp in length, in less than 24 hours and with essentially no introduced errors. The error filter will be integrated with the massively parallel, light-directed DNA synthesis technology of the existing Maskless Array Synthesizer (MAS) and a light-directed oligonucleotide release chemistry developed by GA founders at the University of Wisconsin-Madison, and licensed to GA. Specific aims for the project include: attachment of an error binding protein to a solid support, determination of binding capacity and optimal binding conditions of the error filter, and determination of the optimal error filtering methodology. The ability to produce error-free long DNA fragments will revolutionize the field of molecular biology. It will allow the production of custom designed DNA for uses in protein engineering, DNA vaccines, gene therapy, and other research related to improving human health. This ability will significantly reduce the scientific community's reliance on large collections of cloned DNA fragments now used in genetic research, since almost any DNA of known sequence could be synthesized on demand. This technology will greatly accelerate the pace of genetic research and lead to the development of new drugs, treatments for genetic diseases and advanced genetic technologies.