Nanofabricated Particles for Biolistic Delivery

描述(由申请人提供)： 我们建议开发多功能复合颗粒，作为生物应用中改进的DNA输送载体。这些颗粒将通过逐层技术使用纳米颗粒、聚合物和生物分子的受控组装来制造，其中核心和每一后续层将特定的功能添加到最终颗粒中。密度、稳定性、表面积和表面化学成分将由核心层和周围壳层的材料和尺寸的选择来控制。当这些新的纳米制造设备用于生物应用时，该过程的效率和重复性将大大提高。这些新颖的纳米粒子将引起学术和商业研究领域中使用生物基因转移系统的用户的广泛兴趣。

DESCRIPTION (provided by applicant): We propose the development of multi-functional composite particles for use as improved DNA delivery vehicles in biolistic applications. These particles will be fabricated using the controlled assembly of nanoparticles, polymers, and biomolecules via a layer-by-layer technique where the core and each subsequent layer add specific functionality to the final particle. The density, stability, surface area, and surface chemical composition will be controlled by the choice of material and dimensions of the core and surrounding shell layers. When these novel nano-fabricated devices are used in biolistic applications, the efficacy and reproducibility of the procedure will be greatly enhanced. These novel nanoparticles will be of broad interest to users of biolistic transfection systems in both academic and commercial research communities.