HEMATOPOIETIC GROWTH FACTORS IN A CANINE STEM CELL MODEL

它旨在产生对干细胞生理学的新见解， 通过实现植入，提高供体的可用性和安全性， 犬白细胞移植物抗宿主病（GVHD）不存在 抗原（DLA）半相合和匹配的供体细胞。 第二 该领域旨在了解生长因子 可以改变全身照射对产生 粒细胞和血小板。 具体目标是：（1）明确 犬造血祖细胞的表型存在于 用生长因子动员外周血并测定其体外活性 和体内功能; 2）建立成功的干细胞移植物， 在基因组学上与DLA相同或 与细胞供体是DLA-单倍同一的;和3）鉴定 生长因子或组合是最有效的I修改骨髓 全身照射的毒性，并确定其机制 负责这项活动。

It is aimed at generating new insights into stem cell physiology and increasing donor availability and safety by achieving engraftment in the absence of graft vs. host disease (GVHD) with dog leukocyte antigen (DLA) haploidentical and matched donor cells. The second area is aimed at understanding the mechanisms whereby growth factors can modify the effects of total body irradiation on production of granulocytes and plateletes. The specific aims are: (1) define the phenotypes of canine hematopoietic progenitors present in the peripheral blood mobilized with growth factors and assay their in vitro and in vivo functions; 2) establish successful stem cell grafts without GVHD in canine litermates which are genotopically DLA-identical or are DLA-haploidentical with the cell donor; and 3) identify which growth factors or combinations are most effective I modifying marrow toxicity of total body irradiation and define the mechanisms responsible for this activity.