Agincourt II Nanovolume Protein Crystallization Robot

Agincourt II 纳米体积蛋白质结晶机器人

• 批准号: 6742727
• 负责人: KEN GOODWILL
• 金额: $ 65.82万
• 依托单位: SYRRX, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2005-08-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6742727
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; combinatorial chemistry; computer program /software; crystallization; high throughput technology; nanotechnology; protein purification; protein structure; proteomics; robotics; technology /technique development; tissue /cell culture

DESCRIPTION (provided by applicant): Syrrx Inc. is developing and marketing a complete High Throughput Structural Biology (HTSB) factory that streamlines crystal structure efforts and accelerates the process of pharmaceutical drug discovery. Enabled by Syrrx's patented Nanovolume CrystailizationTM technology, the HTSB system achieves high throughput rates with a substantial reduction in the amount of sample consumed. This HTSB factory comprises modules for protein expression in both bacterial and baculovirus systems and for automated protein extraction and purification, as well as for preparing crystallization screening solutions, and performing the actual nanovolume sitting-drop crystallization experiment with the Agincourt(TM) crystallization robot. Downstream modules include automated crystal imaging FortsTM to store and monitor crystal growth, with all of the modules and the complete system managed and controlled by a robust and specially designed Laboratory Information Management System (LIMS). Syrrx has secured a partnership with Robotic Technology Systems (RTS) Life Sciences to design and build this state-of-the art integrated system for protein crystallography. RTS has extensive experience in engineering and software design for high throughput screening and automated cell culture systems. Syrrx is working closely with RTS to use our experience to build the next generation robotic crystallization laboratory. Although crystallization robots have been built and commercialized before, the Syrrx system has a much higher throughput, true integration of all steps from protein solution to data quality crystal, patented nanovolume technology, and sophisticated software that allow scientists to think about the experiment and not how to operate the machine.

描述（由申请人提供）：Syrrx Inc.正在开发和销售一个完整的高通量结构生物学（HTSB）工厂，简化晶体结构的工作，加快药物发现的过程。通过Syrrx的专利Nanovolume CrystailizationTM技术，HTSB系统实现了高吞吐率，大大减少了样品消耗量。该HTSB工厂包括用于在细菌和杆状病毒系统中进行蛋白质表达的模块、用于自动化蛋白质提取和纯化的模块、用于制备结晶筛选溶液的模块以及用于使用Agincourt（TM）结晶机器人进行实际的纳米体积坐滴结晶实验的模块。下游模块包括用于存储和监控晶体生长的自动晶体成像FortsTM，所有模块和完整的系统都由一个强大的专门设计的实验室信息管理系统（LIMS）进行管理和控制。Syrrx已与机器人技术系统（RTS）生命科学公司建立了合作伙伴关系，以设计和构建这种最先进的蛋白质晶体学集成系统。RTS在高通量筛选和自动化细胞培养系统的工程和软件设计方面拥有丰富的经验。Syrrx与RTS密切合作，利用我们的经验建立下一代机器人结晶实验室。虽然结晶机器人之前已经被建造和商业化，但Syrrx系统具有更高的吞吐量，真正集成了从蛋白质溶液到数据质量晶体的所有步骤，专利纳米体积技术和复杂的软件，使科学家能够思考实验而不是如何操作机器。