Novel Racemases for Enzymatic Dynamic Kinetic Resolution

描述（由申请人提供）：水解酶是有价值的工业生物催化剂，用于制备光学纯化合物。尽管有优势，但这些酶对外消旋底物的最大单次产物收率限制在50%。如果在动态动力学分辨率下与水解酶一起原位使用，底物特异性消旋酶提供了实现100%产率而不回收的潜力。然而，很少有工业上合适的消旋酶的例子被描述。

DESCRIPTION (provided by applicant): Hydrolases are valuable industrial biocatalysts used to prepare optically pure compounds. Despite advantages, these enzymes are restricted to a maximum single pass product yield of 50% with racemic substrates. Substrate specific racemases offer the potential to achieve 100% yield without recycling if used in situ with hydrolases in dynamic kinetic resolutions. However, few examples of industrially appropriate racemases have been described. Ingenza proposes to use in vivo selections to isolate and develop novel racemases and establish dynamic kinetic resolution as a more general method for synthetic process chemistry. The initial focus will be the isolation of N-acylamino acid racemases for use with L- or D-aminoacylases to prepare unnatural amino acids and amines. Such compounds are requisites as synthons for antiviral and antineoplastic drugs. New pharmaceuticals from anticipated advances in proteomics will likely increase demand for this technology. Ingenza proposes to develop a toolbox of racemases and an efficient, scaleable technologyfor use in organic synthetic reactions. Phase I will show feasibility of the selective approach in isolating novel racemases. In Phase II we will optimize enzyme properties and application as applied biocatalysts. In Phase III, we will commercialize the technology for use by synthetic organic chemists and pharmaceutical manufacturers.