Core Grant for Vision Research - Core 1

核心1：眼组学和定量分子生物学 摘要 Core 1的目标是向具有合格赠款和其他愿景的用户提供以下服务 研究人员定量分子生物学服务包括提供咨询， 使用定量PCR，使用酶标仪定量抗体、细胞因子， 其他分析物，使用分光光度计和RNA、DNA和蛋白质样本的生物测定仪， 激光显微切割系统从组织切片中获得材料用于转录组学， 代谢组学或蛋白质组学分析，正常和转基因的等位基因表征 小鼠，核酸探针设计，原位杂交，定量报告基因分析。这一核心 还将促进校园资源的使用，如生物技术中心（NextGen测序， RNAseq、数据处理和分析、蛋白质组学等）和流式细胞仪

CORE 1: OCULAR OMICS AND QUANTITATIVE MOLECULAR BIOLOGY ABSTRACT The goal of Core 1 is to provide the following services to users with qualifying grants and other vision researchers. The quantitative molecular biology services include providing consultation for and access to quantitative PCR, access to plate readers for quantitation of antibodies, cytokines, and other analytes, access to spectrophotometers and a bionalazyer for RNA, DNA and protein samples, a laser microdissection system to obtain material from tissue sections for transcriptomics, metabolomics, or proteomics analysis, allelic characterization of normal and genetically modified mice, nucleic acid probe design, in situ hybridization, quantitative reporter gene analysis. This core will also facilitate use of campus resources such as the Biotechnology Center (NextGen Sequencing, RNAseq, data handling and analysis, proteomics, etc.) and the Flow Cytometry Facility.