Development of high-quality reference genomes for Anopheles squamosus and An. cydippis

开发鳞状按蚊和按蚊的高质量参考基因组。

• 批准号: 10725180
• 负责人: Yoosook Lee
• 金额: $ 6.27万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-23 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10725180
• 关键词: Acceleration; Accounting; Address; Adult; Anopheles Genus; Area; Beds; Behavior; Biological Assay; Bite; California; Chromosomes; Collection; Complex; Conserved Sequence; Culicidae; DNA Library; DNA sequencing; Data; Development; Diagnostic; First Generation College Students; Florida; Foundations; Genbank; Genes; Genetic; Genetic Polymorphism; Genome; Genomics; Genotype; Goals; Human; Hybrids; Improve Access; Individual; Insecticide Resistance; Insecticides; Larva; Life; Link; Malaria; Manuals; Mitochondria; Modeling; Morphology; Orthologous Gene; Oxidases; Parasites; Phenotype; Plasmodium falciparum; Population; Predisposition; Procedures; Process; Pupa; Rapid diagnostics; Rapid screening; Research; Residual state; Risk; Sampling; Scientist; Siblings; Southern Africa; Sporozoites; Training; Trust; Universities; Vector Ecology; Work; Zambia; barrier to testing; contig; cytochrome c oxidase; design; diagnostic assay; epidemiology study; evidence base; experience; functional genomics; genetic information; genome resource; improved; infection rate; malaria infection; malaria transmission; model organism; neglect; preference; reference genome; scaffold; sequencing platform; therapeutically effective; trait; transcriptome sequencing; transmission process; vector; vector competence; vector control; vector mosquito

Project Summary Despite the tremendous effort to minimize the impacts of malaria in southern Africa, it has yet to be eliminated from this region. Many understudied mosquito vector species inhabit this area and may be perpetuating malaria transmission in this pre-elimination zone. Anopheles squamosus is one of the most abundantly caught mosquito species in southern Zambia. This species is also frequently infected with Plasmodium falciparum, a causal agent of human malaria, leading to the hypothesis that it is a critical vector of malaria transmission in Zambia. A major barrier of testing this hypothesis and studying An. squamosus is the lack of genetic information. In addition to this barrier, An. squamosus is indistinguishable from An. cydippis in the adult stage, making it difficult to establish links between genotypes to phenotypes important for parasite transmission and vector control, including vector competence, insecticide resistance, and host preference. This study addresses these two key barriers by (1) producing high-quality genomes of An. squamosus and An. cydippis, (2) identifying genetic polymorphisms within An. squamosus, and (3) producing gene annotation of An. squamosus genome. The genome of An. squamosus will facilitate the identification of species-specific markers for a diagnostic assay that can be used to distinguish this species from other mosquitoes. Our project will catalyze the study of An. squamosus and other understudied malaria vectors, as well as their impacts on malaria transmission. The new genomic resources for An. squamosus can be leveraged for studying mosquito vector behavior and devise effective control strategies of understudied malaria vectors in pre-elimination settings.

项目摘要 尽管为最大限度地减少疟疾对南部非洲的影响作出了巨大努力，但尚未消除疟疾。 来自这一地区。许多未被研究的蚊媒物种栖息在这一地区，并可能继续存在。 疟疾在这一消灭前区域的传播。鳞片按蚊是被捕获最多的物种之一。 赞比亚南部的蚊子种类。这个物种还经常感染恶性疟原虫，一种 人类疟疾的病原体，导致假设它是疟疾传播的关键媒介 赞比亚。检验这一假说和研究AN的一个主要障碍。鳞毛虫是缺乏遗传的 信息。除了这个障碍，还有一个。鳞鳞鱼和鳞鳞鱼没有什么区别。鲤鱼在成虫阶段， 使得很难在基因类型和对寄生虫传播至关重要的表型之间建立联系 媒介控制，包括媒介能力、杀虫剂抗性和寄主偏好。这项研究致力于 这两个关键障碍是通过(1)生产高质量的An基因组。鳞毛虫和An.鲤鱼(2) 确定一个基因的多态。(3)产生An的基因注释。 鳞毛虫基因组。An的基因组。鳞毛虫将促进物种特异性标记的识别 一种可以用来区分这种蚊子和其他蚊子的诊断方法。我们的项目将 促进了对AN的研究。鳞毛虫和其他未被充分研究的疟疾媒介及其对 疟疾传播。为一种新的基因组资源。鳞毛虫可以用来研究蚊子 消灭前未被研究的疟疾媒介的行为和有效控制策略 设置。