Alcohol Dependence And Chromosome 11p15.5

为了追踪11p15.5染色体与酒精依赖的连锁关系，我们创建了两个用于半自动荧光基因分型的短串联重复标记面板，其中包含分布在11p15.5染色体远端20 cM的15个位点。一个小组（6个基因座）已经在大约500名来自西南美洲印第安部落的精神病学访谈个体中进行了键入。第二个面板（9个位点）已经优化，输入将很快开始。此外，DRD4（一个与酒精依赖易感相关的候选基因）的两个编码多态性和一个启动子多态性已经被分型，以便在该基因座上形成单倍型，该基因座远高于全基因组连锁扫描中链接的短串联重复位点。利用这些标记构建的单倍型进行连锁，证实了在第二组西南美洲印第安人同胞对中发现的主要连锁，表明导致酒精依赖的位点位于11p15.5区域。

In order to follow-up a linkage finding on chromosome 11p15.5 to alcohol dependence, two short tandem-repeat marker panels for semi-automated fluorescent genotyping containing 15 loci distributed over the distal 20 cM of chromosome 11p15.5 have been created. One panel (six loci) has been typed in approximately 500 psychiatrically-interviewed individuals from a Southwest American Indian tribe. A second panel (nine loci) has been optimized and typing will begin soon. In addition, two coding polymorphisms and one promoter polymorphism at DRD4, a candidate gene for involvement in vulnerability to alcohol dependence, have been typed in order to develop haplotypes at this locus, distal to the short-tandem repeat locus linked in the whole genome linkage scan. Linkage using haplotypes constructed from these markers has confirmed the primary linkage finding in a second panel of Southwest American Indian sib pairs, indicating that a locus contributing to alcohol dependence is in the 11p15.5 region.