An enzyme-based assay for the detection of acetaldehyde-protein adducts

摘要 长期饮酒是世界范围内的一个重要问题，可能导致成瘾和损害 在整个身体。酒精代谢的第一步是将其转化为乙醛，这是一种高度 活性和毒性分子。乙醛与蛋白质和DNA加合物的形成是主要的 乙醇毒性机制乙醛加合物是酒精滥用的直接生物标志物。我们打算 开发一种简便快速的酶法测定乙酰丙酮-蛋白质加合物，以评估酒精含量 使用和与酒精相关的健康后果。

ABSTRACT Chronic alcohol consumption is a significant issue worldwide which can lead to addiction and damage throughout the body. The first step in alcohol metabolism is its conversion into acetaldehyde, which is a highly reactive and toxic molecule. The formation of acetaldehyde adducts with protein and DNA is a primary mechanism of ethanol toxicity. Acetaldehyde adducts are a direct biomarker for alcohol abuse. We intend to develop a facile and rapid enzyme-based assay for measuring acetaldehyde-protein adducts to assess alcohol use and alcohol-related health consequences.