Nanoprobes and integrated nanodevices for cancer detection and treatment

用于癌症检测和治疗的纳米探针和集成纳米器件

• 批准号: 7547531
• 负责人: WOUNJHANG PARK
• 金额: $ 2.84万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-09 至 2009-09-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7547531
• 关键词: BRAF gene; Biological Markers; Blood specimen; Cancer Biology; Cancer Detection; Cells; Clinic; Clinical; Collaborations; DNA; Detection; Development; Diagnostic; Disease; Disease Management; Early Diagnosis; Electrical Engineering; Environment; Family history of; Goals; Hand; Image; Indium; Injection of therapeutic agent; Intake; Investigation; Knowledge; Lead; Malignant Neoplasms; Melanocortin 1 Receptor; Melanoma Cell; Metals; Metastatic Melanoma; Methods; Microscopic; Molecular Profiling; Mutation; Nanotechnology; Oncologist; Optics; Patients; Phase; Process; Public Health; Purpose; RNA; Regulatory Pathway; Research; Sampling; Scheme; Screening for cancer; Signal Transduction; Solutions; Specificity; System; Techniques; Technology; Training; Training Programs; Work; base; desire; experience; frontier; inhibitor/antagonist; melanocyte; melanoma; nano; nanodevice; nanoparticle; nanoprobe; nanoscale; nanoshell; novel therapeutics; optical imaging; tool

DESCRIPTION (provided by applicant): Malignant melanoma is the most rapidly increasing cancer throughout the world. Current methods for detection of metastatic melanoma are not adequate. At least 20% of patients develop advanced disease, which is rarely curable. In this application, we propose a training program for an electrical engineer who is an expert in nanotechnology so that he can better understand the clinical needs for early detection of cancer, especially melanoma. This training experience will establish the applicant as a truly interdisciplinary expert well versed in both cancer biology and nanotechnology. It will also establish a lasting collaboration between the applicant and the sponsors that will explore the new frontiers of cancer nanotechnology. The training will have two phases: (1) understanding and identifying DNA and RNA biomarkers for melanoma and (2) developing nanotechnology platform for detection and manipulation of the biomarkers. The biomarkers to be investigated include BRAF mutations, melanocortin 1 receptor mutations, and microRNA expression. We will study the expression levels and their effects in melanoma cells and normal melanocytes. A particular emphasis will be placed on understanding their effects in conjunction with the nanoprobes to be developed concurrently. The nanoscale probes will be optimized for rapid, high-specificity detection of melanoma cells at extremely low concentrations. Specifically, we will investigate new synthesis and conjugation techniques for nanoprobes which will include noble metal nanoparticles, nanorods and nanoshells. Using these probes, we will investigate two technology platforms: (1) fluidic-based technique in which nanoprobes are suspended and interact with other biomolecules in solution (e.g., blood sample); and (2) optical imaging and targeting by nanoprobe injection into melanoma cells. For (1), we will develop a highly integrated nano- and micro-fluidic system in which a microscopic amount of sample is accurately manipulated and examined for high-sensitivity detection and imaging. For (2), we will investigate in detail the intake process of nanoprobes by various cells and the dynamics and interaction of nanoprobes inside the cell. RELEVANCE TO PUBLIC HEALTH: Malignant melanoma is the most rapidly increasing cancer throughout the world and at least 20% of melanoma patients develop advanced disease which is rarely curable. Methods for early detection is therefore a high priority and new strategies for effective treatment and management of the disease are highly desired. The proposed training program seeks to apply nanotechnology to the detection and treatment of melanoma cells.

描述（由申请人提供）：恶性黑色素瘤是全世界增长最快的癌症。目前用于检测转移性黑色素瘤的方法是不够的。至少有20%的患者发展为晚期疾病，这是很少可以治愈的。在这个应用程序中，我们提出了一个培训计划的电气工程师谁是纳米技术的专家，使他能够更好地了解癌症，特别是黑色素瘤的早期检测的临床需求。这种培训经验将使申请人成为真正的跨学科专家，精通癌症生物学和纳米技术。它还将在申请人和赞助商之间建立持久的合作，探索癌症纳米技术的新前沿。培训将分为两个阶段：（1）理解和识别黑色素瘤的DNA和RNA生物标志物，以及（2）开发用于检测和操作生物标志物的纳米技术平台。待研究的生物标志物包括BRAF突变、黑皮质素1受体突变和microRNA表达。我们将研究其在黑色素瘤细胞和正常黑素细胞中的表达水平及其作用。一个特别的重点将放在理解他们的影响与纳米探针同时开发。纳米探针将被优化用于在极低浓度下快速，高特异性检测黑色素瘤细胞。具体来说，我们将研究新的合成和共轭技术的纳米探针，其中将包括贵金属纳米粒子，纳米棒和纳米壳。使用这些探针，我们将研究两个技术平台：（1）基于流体的技术，其中纳米探针悬浮并与溶液中的其他生物分子相互作用（例如，血液样品）;和（2）光学成像和通过纳米探针注射到黑素瘤细胞中的靶向。对于（1），我们将开发一种高度集成的纳米和微流体系统，在该系统中，可以精确地操作和检查微量样品，以实现高灵敏度的检测和成像。对于（2），我们将详细研究各种细胞对纳米探针的摄入过程以及纳米探针在细胞内的动力学和相互作用。 与公共卫生的关系：恶性黑色素瘤是世界上增长最快的癌症，至少20%的黑色素瘤患者发展为晚期疾病，很少能治愈。因此，用于早期检测的方法是高度优先的，并且高度期望用于有效治疗和管理该疾病的新策略。拟议的培训计划旨在将纳米技术应用于黑色素瘤细胞的检测和治疗。