Methylation analysis of the gene ADRB2

ADRB2基因甲基化分析

• 批准号: 7588589
• 负责人: GREGORY A HAWKINS
• 金额: $ 7.4万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-19 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7588589
• 关键词: ADRB2 gene; Agonist; Asthma; Binding; Candidate Disease Gene; Cell Proliferation; Cells; Chronic Obstructive Airway Disease; Code; DNA; DNA Methylation; Data; Epigenetic Process; Future; Gene Expression; Gene Expression Regulation; Gene Proteins; Genes; Genetic; Genetic Polymorphism; Genotype; Health; Human; Incubated; Investigation; Measures; Messenger RNA; Methylation; Monitor; Obstructive Lung Diseases; Pattern; Pharmacogenetics; Pilot Projects; Proliferating; Proteins; Reverse Transcriptase Polymerase Chain Reaction; Role; Site; Smooth Muscle Myocytes; Tachyphylaxis; Testing; Time; base; cell type; exposed human population; field study; promoter; protein expression; radioligand; receptor; receptor expression; research study; respiratory smooth muscle; response

Epigenetic regulation of gene expression is an emerging concept relevant to human health. This proposal's objective is to investigate the epigenetic control of ADRI32 expression in cells relevant to obstructive lung .=. �'m exposed to a a-agonist, the degree of DNA methylation is increased in the promoter and coding region of the gene for the 13-agonist receptor, ADRB2. Based on the classical function that DNA methylation blocks or decreases gene expression, we hypothesize that long term exposure of HASM cells to 13-agonist increases methylation of ADRB2 and resulting in decreased expression of ADRB2. This decrease in ADR132 could be a major mechanism contributing to tachyphylaxis to 13-agonist treatment in asthma and COPD. In this proposed pilot study, we will test our hypothesis by: 1) Performing methylation specific sequencing on ADRI32 to localize and quantitate methylated CpG sites in DNA isolated from HASM cells exposed to 13-agonists; 2) Assessing the effects of ADR2 methylation on gene expression. v+3 �:3 W-0 =:r a:' �--, any 0-0 disease. We have recent preliminary data showing that in human airway smooth muscle (HASM) cells U).2 M-0 .4) `Z-_ Gam. pro. -0a� >,Q tom/! a--

基因表达的表观遗传调控是一个与人类健康相关的新兴概念。本研究的目的是研究ADRI32在阻塞性肺相关细胞中表达的表观遗传控制