Research Core: Biochemistry and histology/histomorphometry

研究核心：生物化学和组织学/组织形态计量学

• 批准号: 7684866
• 负责人: Caren Gundberg
• 金额: $ 39.41万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7684866
• 关键词: Alkaline Phosphatase; Animal Model; Animals; Basic Science; Biochemical; Biochemical Markers; Biochemistry; C-telopeptide; C-terminal; Calcium; Cells; Collagen Type I; Creatinine; Goals; Histology; Homeostasis; Hormones; Human; Measurement; Metabolism; Minerals; Mus; Musculoskeletal; Musculoskeletal Diseases; Osteocalcin; Phosphorus; Physiology; Preparation; Research; Research Project Grants; Serum; Services; Skeletal system; Slide; Staining method; Stains; Standards of Weights and Measures; TdT-Mediated dUTP Nick End Labeling Assay; Tissues; Tolonium chloride; Translational Research; Urine; alizarin; bone; bone turnover; glutamyl-lysyl-alanyl-histidyl-aspartyl-glycyl-glycyl-arginine; human study; sample fixation; urinary

The Research Core will be directed by Dr. Caren Gundberg. This Core will provide direct skeletal analysis and biochemical measurements of bone and mineral metabolism as to facilitate CORT basic and translational research projects. The Core will partner with the Physiology Core of the Yale Core Center for Musculoskeletal Diseases, and allow for the necessary expansion of services in order to provide the dedicated support of CORT projects. Specific services offered will include: 1. Fixation, embedding, sectioning, and slide preparation of undecalcified skeletal tissue. 2. Specialized staining of bone from animal models, including toluidine blue, von Kossa, Safronin O, alkaline phosphatase as well as specific immunohistochemical localizations and TUNEL assays. 3. Measurement and interpretation of standard static and dynamic parameters of bone histomorphometrical analysis. 4. Assessment of mineralizing cells in culture. Analyses will include both alizarin red and von Kossa staining of mineralizing cultures as well as direct measurement of calcium and phosphorus content, alkaline phosphatase and osteocalcin. 5. Measurement of PTH (intact and mid-molecule), FGF23 (intact and C-terminal), 25(OH)D, 1,25(OH)2D, serum PINP, human osteocalcin, C-telopeptide of type I collagen (Crosslaps) and urinary minerals and creatinine will be provided for human studies. 6. Measurement of mouse PTH, FGF23 (intact), 25(OH)D, and 1,25(OH)2D, mouse osteocalcin, Ctelopeptide of type I collagen (Ratlaps), and urinary minerals will be provided for human studies.

研究核心将由 Caren Gundberg 博士领导。该核心将提供直接的骨骼分析 骨和矿物质代谢的生化测量，以促进 CORT 基本和 转化研究项目。该核心将与耶鲁大学核心中心的生理学核心合作 肌肉骨骼疾病，并允许必要的服务扩展，以便提供 CORT 项目的专门支持。提供的具体服务将包括： 1. 未脱钙骨骼组织的固定、包埋、切片和玻片制备。 2. 对动物模型骨进行专门染色，包括甲苯胺蓝、von Kossa、Safronin O、 碱性磷酸酶以及特异性免疫组织化学定位和 TUNEL 测定。 3. 骨标准静态和动态参数的测量和解释 组织形态计量学分析。 4. 培养物中矿化细胞的评估。分析将包括茜素红和 von Kossa 矿化培养物染色以及直接测量钙和磷含量，碱性 磷酸酶和骨钙素。 5. PTH（完整和中间分子）、FGF23（完整和 C 末端）、25(OH)D、1,25(OH)2D、 血清PINP、人骨钙素、I型胶原C端肽（Crosslaps）和尿矿物质 肌酐将用于人体研究。 6. 小鼠 PTH、FGF23（完整）、25(OH)D 和 1,25(OH)2D、小鼠骨钙素、Ctelopeptide 的测量 I 型胶原蛋白 (Ratlaps) 和尿液矿物质将用于人体研究。