AFFERENT REGULATION OF CHOLINERGIC FOREBRAIN NEURONS

胆碱能前脑神经元的传入调节

• 批准号: 7638767
• 负责人: LASZLO ZABORSZKY
• 金额: $ 3万
• 依托单位: RUTGERS THE STATE UNIV OF NJ NEWARK
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 2010-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7638767
• 关键词: Address; Alzheimer' s Disease; Area; Attention; Axon; Behavior; Body part; Brain region; Cells; Cerebral cortex; Chemicals; Cholinergic Agents; Chromosome Pairing; Classification; Cognitive; Collaborations; Complex; Computer information processing; Data; Deposition; Disease; Drug abuse; Electroencephalography; Electron Microscopy; Electrons; Electrophysiology (science); Evoked Potentials; Face; Fire - disasters; Fluoro-Gold; Glutamate Transporter; Glutamates; Grant; Hungary; In Situ Hybridization; Injection of therapeutic agent; Interneurons; Label; Light; Localized; Location; Memory; Messenger RNA; Methods; Microscopic; Motor; Neurons; Numbers; Parvalbumins; Play; Prefrontal Cortex; Process; Property; Prosencephalon; Regulation; Research Personnel; Role; Schizophrenia; Sensory; Site; Staining method; Stains; Synapses; System; Techniques; Testing; Tracer; Work; basal forebrain; basalt; biocytin; calretinin; cholinergic; cholinergic neuron; college; medical schools; neuropsychiatry; postsynaptic; programs; research study; response; segregation; somatosensory; somesthesis

The basal forebrain (BF), a complex brain region that contains cholinergic and various non-cholinergic corticopetal neurons and interneurons, plays an important role in modulating cortical activity. Corticopetal BF projections, especially the cholinergic component, have been implicated in memory, attention, and many other cognitive processes. While the cholinergic corticopetal component received particular emphasis, very little is known about non-cholinergic components of the corticopetal projection, with special reference to putative glutamatergic neurons, and the local information processing in the BF. In this proposal we will investigate: 1) whether or not the neuronal subtypes of the BF include cortically projecting glutamatergic cells, 2) what the local target(s) of the axon collaterals of NPY interneurons are. 3) whether or not local NPY-containing neurons also receive prefrontal input. In these experiments morphological (single cell staining, immunocytochemical marking, in situ hybridization and anatomical tracing, both at the light and electron microscopical levels) will be combined with electrophysiological (single unit and EEG) techniques. In additional studies, we will investigate, in a systematic fashion, how architectural features at the network level would enable the basal forebrain corticopetal system to participate in coordinating association and sensory- motor functions. For example, we will test the hypothesis that neurons in the BF which project to interconnected somatosensory ($1, $2) and motor cortical areas (M1), and which cooperate with each other during somesthesis-guided behaviors, show larger overlap than projections to neighboring cortical areas that, in turn, represent non-contiguous body-parts. In these experiments, we will use dual retrograde tracer injections into electrophsyiologically identified sensory or motor cortical representations and retrogradely labeled cholinergic and non-cholinergic neurons in the BF will be analyzed for segregation or overlap. These studies will have significant impact on our understanding of information processing in basalo-cortical networks, and concomitantly the understanding of the aberrant information processing in these networks that characterize several neuropsychiatric disorders, including Alzheimer's disease, schizophrenia, and drug abuse.

基底前脑（BF）是一个复杂的脑区，含有胆碱能和各种非胆碱能神经元。 皮层神经元和中间神经元在调节皮层活动中起重要作用。皮质BF 投射，特别是胆碱能成分，与记忆、注意力和许多其他功能有关。 其他认知过程。虽然胆碱能皮质成分得到特别强调， 关于皮质脑顶投射的非胆碱能成分知之甚少，特别是关于 推测的丘脑能神经元，以及BF中的局部信息处理。在本提案中，我们将 研究：1）BF的神经元亚型是否包括皮质投射的神经元， 2）NPY中间神经元轴突侧支的局部靶点。3)无论是否本地 含有NPY的神经元也接受前额叶输入。在这些实验中，形态学（单细胞 染色，免疫细胞化学标记，原位杂交和解剖示踪，无论是在光和 电子显微镜水平）将与电生理学（单个单位和EEG）技术相结合。在 另外的研究，我们将调查，在一个系统的方式，如何在网络层面的架构功能， 将使基底前脑皮质脑系统参与协调协会和感觉- 运动功能例如，我们将测试BF中投射到 相互连接的躯体感觉区（$1，$2）和运动皮层区（M1），它们相互合作 在躯体感觉引导的行为中，显示出比邻近皮层区域的投射更大的重叠 其又表示非邻接身体部分。在这些实验中，我们将使用双逆行示踪剂 注射到电生理学鉴定的感觉或运动皮层代表中， 将分析BF中标记的胆碱能和非胆碱能神经元的分离或重叠。这些 研究将对我们理解基底皮层的信息处理产生重大影响 网络，并伴随着对这些网络中异常信息处理的理解， 描述了几种神经精神疾病，包括阿尔茨海默病，精神分裂症和药物 虐待