Surgical and Animal Core

我们实验室的一个标志是开发新颖和创新的体内肿瘤模型，使 我们研究治疗药物输送的障碍。这是可能的，因为出色的手术 我们实验室拥有专业知识和独特的动物设施，该核心是其基础。这个 核心将继续发挥两项功能：(i) 手术支持，其中包括新型动物模型 (ii) 动物的培育和饲养。两者对于成功完成所有任务都至关重要 项目目标。因此，这个核心构成了我们 PPG 竞争更新的基石。该核心将 继续为本PPG的所有项目建立并提供动物模型。透明窗模型 - 乳腺脂肪垫窗口（所有三个项目）、颅骨窗口（项目 1、2）和背部皮肤 腔室（项目 3）- 能够对分子、细胞、解剖学和 分别在乳腺癌、脑癌和皮肤癌的原位器官环境中的功能参数。在 此外，自发性肿瘤使我们能够研究宿主微环境在肿瘤中的作用 发展和治疗。该核心维护的 Cox-7 限生动物群体使我们能够 在免疫缺陷小鼠中进行纵向生理学研究。这些研究极其困难 而其他地方的成本更高。该设施将继续为我们提供统一的实验动物 不含鼠科病毒、病原菌和寄生虫的品质。这使我们能够执行 不使用抗生素的外科手术。定义菌群 C3H、FVB、C57BL/6、Nude、SCID、rag-T'' rag-T'-、VTGFP-GFP/（FVB、C3H、rag-Z'' 背景）、Tie2p-GFP/（FVB、rag-T'- 背景）、EFlcf- GFP/FVB 和 ACTbp-GFP/C57VL/6 小鼠目前可在 Cox-7 设施中使用。附加 所有项目的转基因和基因敲除小鼠也将被改造或重新衍生、繁殖和维持 适用于设施内的所有项目。经过筛选且不含小鼠病原体的肿瘤将被连续进行 在体内传代并植入到群体内的实验动物中。体内肿瘤不会 传代超过第五代（F5）以避免肿瘤特征的变化。这保证了紧 控制动物和肿瘤的质量。 Core C还将继续提供控释泵 植入、组织样本采集、血管导管放置和术后护理。

A hallmark of our laboratory is the development of novel and innovative in vivo tumor models that allow us to study barriers to the delivery of therapeutic agents. This is possible due to the outstanding surgical expertise and unique animal facility available in our lab, for which this Core serves as its foundation. This Core will continue to serve two functions: (i) surgical support which includes novel animal model development, and (ii) breeding and maintenance of animals. Both are vital for successful completion of all project goals. Thus, this Core forms a cornerstone of the competing renewal of our PPG. This Core will continue to establish and provide animal models to all Projects of this PPG. Transparent window models - the mammary fat pad window (all three Projects), the cranial window (Projects 1, 2) and the dorsal skin chamber (Project 3)- enable in vivo time-course monitoring of molecular, cellular, anatomical, and functional parameters in orthotopic organ environments for breast, brain and skin cancers, respectively. In addition, spontaneous tumors allow us to study the role of the host microenvironment in tumor development and treatment. The Cox-7 gnotobiotic animal colony maintained by this Core allows us to carry out longitudinal physiological studies in immunodeficient mice. These studies are extremely difficult and more costly elsewhere. This facility will continue to provide us with experimental animals of uniform quality that are free of murine viruses, pathogenic bacteria, and parasites. This enables us to carry out surgical procedures without the use of antibiotics. Defined flora C3H, FVB,C57BL/6, Nude, SCID, rag-T'' rag-T'-, VTGFP-GFP/(FVB, C3H, rag-Z'' background), Tie2p-GFP/(FVB, rag-T'- background), EFlcf- GFP/FVB, and ACTbp-GFP/C57VL/6 mice are currently available in the Cox-7 facility. Additional transgenic and knock-out mice for all Projects will also be engineered or rederived, bred, and maintained for all Projects within the facility. Tumors that are screened and free of mouse pathogens will be serially passaged in vivo and implanted into experimental animals within the colony. In vivo tumors will not be passaged beyond the fifth generation (F5) to avoid changes in tumor characteristics. This assures tight control of the quality of animals and tumors. Core C will also continue to provide controlled-release pump implantation, tissue sample collection, vascular line placement, and post-surgical care.