EFFECT OF AGE ON INSULIN MEDIATED REGULATION OF GHRELIN

年龄对胰岛素介导的生长素释放肽调节的影响

• 批准号: 7606709
• 负责人: RALF M NASS
• 金额: $ 2.41万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7606709
• 关键词: Abdomen; Acute; Age; Amino Acids; Biological Assay; Computer Retrieval of Information on Scientific Projects Database; Eating; Elderly; Fatty acid glycerol esters; Funding; Grant; Homeostasis; Institution; Insulin; Laboratories; Measures; Mediating; Obesity; Octanoic Acids; Peptides; Plasma; Play; Post-Translational Protein Processing; Regulation; Research; Research Personnel; Resources; Role; Somatotropin; Source; Stomach; United States National Institutes of Health; Visceral; age effect; age related; caprylic acid; ghrelin; hydroxyl group; insulin sensitivity

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ghrelin, a peptide which is mainly derived from the stomach, is present in plasma in an active (acylated) and inactive (des-acylated) form. The major active form of ghrelin contains 28 amino acids with a unique post-translational modification: the hydroxyl group of ser3 is esterified by octanoic acid. The n-octanoyl group is essential for the GH (growth hormone) releasing activity. Plasma ghrelin plays a role in energy homeostasis, food intake, and possibly in GH release and obesity. To date, it is unclear how circulating ghrelin levels are regulated and whether there are age-specific differences in the regulation of ghrelin. Insulin sensitivity decreases with age. This is partially a result of the age-dependent increase in abdominal visceral fat. The studies proposed will investigate whether there are differences between young and older adults regarding the acute insulin mediated decrease in circulating ghrelin (bioactive, inactive form) levels. Using assays developed in our laboratory, both bioactive and inactive ghrelin will be measured.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Ghrelin是一种主要来源于胃的肽，以活性（酰化）和非活性（去酰化）形式存在于血浆中。生长激素释放肽的主要活性形式包含28个氨基酸，具有独特的翻译后修饰：ser 3的羟基被辛酸酯化。正辛酰基对于GH（生长激素）释放活性是必需的。血浆ghrelin在能量稳态、食物摄入中起作用，并且可能在GH释放和肥胖中起作用。迄今为止，尚不清楚循环ghrelin水平是如何调节的，以及ghrelin的调节是否存在年龄特异性差异。胰岛素敏感性随着年龄的增长而下降。这部分是由于腹部内脏脂肪的年龄依赖性增加。拟议的研究将调查年轻人和老年人之间是否存在关于急性胰岛素介导的循环ghrelin（生物活性，非活性形式）水平降低的差异。使用我们实验室开发的测定法，将测量生物活性和非活性ghrelin。