THE ROLE OF THE HDL RECEPTOR, SCAVENGER RECEPTOR CLASS B TYPE I, IN HUMAN

HDL 受体（B 类清道夫受体 I 型）在人体中的作用

• 批准号: 7607491
• 负责人: Annabelle Rodriguez
• 金额: $ 0.03万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2007-09-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7607491
• 关键词: Atherosclerosis; Cholesterol Esters; Class; Computer Retrieval of Information on Scientific Projects Database; Estradiol; Fertility; Funding; Genes; Genetic Variation; Genotype; Goals; Grant; HDL receptor; High Density Lipoproteins; Human; Infertility; Institution; Lipids; Measurement; Measures; Mediating; Numbers; Oocytes; Phenotype; Pilot Projects; Population; Pregnancy Outcome; Prevalence; RNA; Research; Research Personnel; Resources; Risk; Role; SR-BI receptor; Source; Testing; Thick; United States National Institutes of Health; Variant; Woman; granulosa cell; macrophage; monocyte; receptor; scavenger receptor; uptake

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this pilot study is to determine the lipid phenotype, SR-BI (scavenger receptor class B type I, HDL receptor) genotypes, and evidence for subclinical atherosclerosis in infertile women. These investigators have recently shown that infertile women with SR-BI RNA levels below the 25th percentile had significantly lower baseline and peak estradiol levels and lower number of retrieved and fertilized oocytes compared to women with SR-BI RNA levels above the 75th percentile. Now they seek to determine the prevalence of SR-BI variants in this population and the association of these variants with the lipid profile, subclinical atherosclerosis and fertility measurements. There are 3 specific aims: 1) To measure the lipid phenotype in infertile women and assess the ability of monocyte-derived macrophage and granulosa cells isolated from these subjects to mediate selective cholesteryl ester uptake from HDL (a functional test of SR-BI, which presumably would be impaired in low expressers compared to high expressers).; 2) To associate alterations in SR-BI expression, function and gene variants with pregnancy outcome measurements and atherosclerotic risk (assessed by intimal wall thickness on carotid doppler) in infertile women. 3) To identify variants within the SR-BI gene in infertile women.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本初步研究的目的是确定不孕妇女的脂质表型、SR-BI（I型B类清道夫受体，HDL受体）基因型和亚临床动脉粥样硬化的证据。这些研究人员最近表明，与SR-BI RNA水平高于第75百分位数的女性相比，SR-BI RNA水平低于第25百分位数的不孕女性具有显著较低的基线和峰值雌二醇水平以及较低的获卵和受精卵数量。现在，他们试图确定SR-BI变体在这一人群中的患病率，以及这些变体与血脂谱、亚临床动脉粥样硬化和生育力测量的关联。 有三个具体目标：1）测量不孕妇女的脂质表型，并评估从这些受试者分离的单核细胞衍生的巨噬细胞和颗粒细胞介导从HDL选择性摄取胆固醇酯的能力（SR-BI的功能测试，与高表达者相比，其可能在低表达者中受损）; 2)在不孕妇女中，将SR-BI表达、功能和基因变异的改变与妊娠结局测量和动脉粥样硬化风险（通过颈动脉多普勒内膜壁厚度评估）相关联。3)确定不育妇女SR-BI基因的变异。