THE ROLE OF THE HDL RECEPTOR, SCAVENGER RECEPTOR CLASS B TYPE I, IN HUMAN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这项先导性研究的目的是确定不孕症妇女的脂质表型、SR-BI(清道夫受体B类I型，高密度脂蛋白受体)基因型别，以及亚临床动脉粥样硬化的证据。这些研究人员最近发现，与SR-BI RNA水平高于第75个百分位数的女性相比，SR-BI RNA水平低于第25个百分位数的不孕不育女性的基线和峰值雌二醇水平显著较低，获得和受精的卵母细胞数量也较少。现在，他们试图确定SR-BI变异体在该人群中的流行率，以及这些变异体与血脂、亚临床动脉粥样硬化和生育测量的关联。该研究有三个具体目标：1)测量不孕妇女的脂质表型，并评估从这些受试者分离的单核细胞来源的巨噬细胞和颗粒细胞介导从高密度脂蛋白中选择性摄取胆固醇酯的能力(SR-BI的功能测试，与高表达者相比，SR-BI可能受到损害)。2)将SR-BI表达、功能和基因变异的变化与妊娠结局测量和不孕不育妇女的动脉粥样硬化风险(通过颈动脉多普勒内膜厚度评估)联系起来。3)确定不孕症妇女SR-BI基因的变异。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this pilot study is to determine the lipid phenotype, SR-BI (scavenger receptor class B type I, HDL receptor) genotypes, and evidence for subclinical atherosclerosis in infertile women. These investigators have recently shown that infertile women with SR-BI RNA levels below the 25th percentile had significantly lower baseline and peak estradiol levels and lower number of retrieved and fertilized oocytes compared to women with SR-BI RNA levels above the 75th percentile. Now they seek to determine the prevalence of SR-BI variants in this population and the association of these variants with the lipid profile, subclinical atherosclerosis and fertility measurements. There are 3 specific aims: 1) To measure the lipid phenotype in infertile women and assess the ability of monocyte-derived macrophage and granulosa cells isolated from these subjects to mediate selective cholesteryl ester uptake from HDL (a functional test of SR-BI, which presumably would be impaired in low expressers compared to high expressers).; 2) To associate alterations in SR-BI expression, function and gene variants with pregnancy outcome measurements and atherosclerotic risk (assessed by intimal wall thickness on carotid doppler) in infertile women. 3) To identify variants within the SR-BI gene in infertile women.