CHARACTERIZATION OF HIV-1 PREINTEGRATION COMPLEX ASSEMBLY AND NUCLEAR TRANSPORT

HIV-1 整合前复合体组装和核运输的表征

• 批准号: 7609844
• 负责人: MICHAEL A BELSHAN
• 金额: $ 18.71万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7609844
• 关键词: Anti-Retroviral Agents; Architecture; Biological Assay; Cell Nucleus; Cells; Class; Complex; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Grant; HIV; HIV-1; In Vitro; Institution; Longevity; Methods; Nuclear Import; Nucleic Acids; Nucleoproteins; Proteins; Research; Research Personnel; Resources; Reverse Transcription; Source; Therapeutic; United States National Institutes of Health; Viral; gel electrophoresis; insight; nucleocytoplasmic transport; size; tandem mass spectrometry; viral DNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The experimental focus of this proposal is to investigate the composition, assembly and transport of the human immunodeficiency virus (HIV) preintegration complex (PIC). The PIC is a large nucleoprotein complex that facilitates transport of the viral DNA into the nucleus of cells upon completion of reverse transcription. The viral components of the PIC have been characterized, but the size of the PIC suggests it contains a large amount of cellular factors, perhaps both protein and nucleic acid. Structural studies of PICs are hampered by several factors, including: 1) Low quantity in the cell; 2) a relatively short life-span; and 3) difficulty of purification. We propose to develop methods to produce and purify a sufficient quantity of PICs to proteomically analyze PIC composition and characterize nuclear transport. Composition will be analyzed by gel electrophoresis and tandem mass spectrometry. Nuclear transport will be assessed by an in vitro nuclear import and integration assay. This data will give insight into the architecture and cellular transport of the PIC, the first step in developing a new class of anti-retroviral therapeutics.

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