Novel Technology for the Detection of Low Abundance Protein

检测低丰度蛋白质的新技术

• 批准号: 7690288
• 负责人: Steingrimur Pall Stefansson
• 金额: $ 10.23万
• 依托单位: CREATV MICROTECH, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-22 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7690288
• 关键词: AIDS Dementia Complex; Affinity Chromatography; Antibodies; Avidin; Biological Assay; Biological Markers; Body Fluids; Caliber; California; Cattle; Central Nervous System Diseases; Chemistry; Chromatography; Clinical; Complex; Computer Systems Development; Computer software; Detection; Detergents; Disease; Evaluation; Force of Gravity; Gases; Generations; Goals; Government; Human; Immobilization; Immunoassay; Laboratories; Left; Ligands; Lipids; Liquid substance; Mass Spectrum Analysis; Measurable; Measures; Mental Health; Methodology; Methods; Microbubbles; Microfluidics; Multiple Sclerosis; Neuraxis; Neurofilament Proteins; Neurons; Parkinson Disease; Peptides; Persons; Phase; Plant Resins; Plasma; Preparation; Procedures; Production; Protein Analysis; Proteins; Reaction; Sampling; Sepharose; Serum; Small Business Innovation Research Grant; Solutions; Solvents; Specimen; Speed; Structure; Surface; Technology; Tissues; Universities; Validation; Vesicle; aqueous; base; cost; design; improved; instrument; magnetic beads; micro-total analysis system; multiplex detection; neurofilament; new technology; novel; pressure; protein complex; public health relevance; tool

DESCRIPTION (provided by applicant): We propose a novel technology for detection of low abundance protein in body fluids, with initial application to a mental health biomarker. One of the most significant challenges facing biomarker discovery is the need to separate high and low abundance proteins for detection of the latter more easily and with precision. The proposed technology is a novel chromatography platform that utilizes unique functionalized micro-structures to capture and purify target analytes from clinical tissue specimens. It does not utilize conventional beads, filters or magnets. We believe that the proposed method will stand out as is a potential breakthrough in the field of sample preparation and will be applied to analysis of numerous low abundance biomarkers. For validation of the proposed technology, this technology will be applied to the detection of neuronal biomarkers. In Phase I of this SBIR, we plan to recover human neurofilament proteins from serum. Because of their specific localization in the central nervous system, the presence of neurofilament proteins outside of this compartment may indicate damage and/or disease of the central nervous system. The presence of neurofilament proteins in body fluids can serve as a sensitive indicator of central nervous system diseases, including multiple sclerosis, Parkinson's disease and HIV dementia. This Phase I SBIR will demonstrate the proof-of-principle detection of neurofilaments as the target analyte in the proposed chromatography technology and design an automated instrument to be constructed in Phase II. PUBLIC HEALTH RELEVANCE: We propose to develop a novel technology for detection of low abundance protein in body fluids, with initial application to a mental health biomarker called neurofilaments. Because of their specific localization in the central nervous system, the presence of neurofilament proteins outside this compartment may indicate damage and/or disease of the central nervous system. The presence of neurofilament proteins in body fluids can serve as a sensitive indicator of central nervous system diseases, including multiple sclerosis, Parkinson's disease and HIV dementia.

描述（由申请人提供）：我们提出了一种检测体液中低丰度蛋白质的新技术，最初应用于精神健康生物标志物。生物标志物发现面临的最重大挑战之一是需要分离高丰度和低丰度蛋白质，以便更容易且精确地检测后者。所提出的技术是一种新型的色谱平台，其利用独特的功能化微结构从临床组织样本中捕获和纯化目标分析物。它不使用传统的珠子，过滤器或磁铁。我们相信，所提出的方法将脱颖而出，是一个潜在的突破，在该领域的样品制备，并将被应用于许多低丰度生物标志物的分析。为了验证所提出的技术，该技术将被应用于检测神经元生物标志物。在SBIR的第一阶段，我们计划从血清中回收人类神经丝蛋白。由于它们在中枢神经系统中的特异性定位，在该隔室之外存在神经丝蛋白可以指示中枢神经系统的损伤和/或疾病。体液中神经丝蛋白的存在可以作为中枢神经系统疾病的敏感指标，包括多发性硬化症、帕金森病和艾滋病毒痴呆症。该第I阶段SBIR将证明在拟议的色谱技术中作为目标分析物的神经丝的原理验证检测，并设计将在第II阶段构建的自动化仪器。 公共卫生关系：我们建议开发一种新技术，用于检测体液中的低丰度蛋白质，并初步应用于称为神经丝的精神健康生物标志物。由于它们在中枢神经系统中的特异性定位，在该隔室之外存在神经丝蛋白可能指示中枢神经系统的损伤和/或疾病。体液中神经丝蛋白的存在可以作为中枢神经系统疾病的敏感指标，包括多发性硬化症，帕金森病和艾滋病毒痴呆症。