SAXS STUDIES OF E COLI WZZ PROTEIN

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Wzz蛋白是一种参与脂多糖（LPS）生物合成的O-抗原组分的链长决定蛋白，也是一种通过掺入不同长度的重复O-单元（O-抗原）而起聚合酶作用的蛋白，其概念与我们对DNA聚合酶的研究相关。 在过去的几年中，已经尝试了各种其他生物物理方法（结晶学、NMR和Cryo-EM）来获得这种膜蛋白wzz的结构信息（Guo，H.等人（2005）Appl. Environ. 微生物学：71，7995-8001）。 三个月前，我们与俄勒冈州立大学的乔治王教授合作，通过SAXS获得其结构信息。 虽然其他方法没有取得重大进展，但我们通过SAXS对这种蛋白质进行了一些非常积极的初步研究。 在提交完整提案之前，我们希望利用这种快速访问来获得更多初步SAXS研究。已经发表了两篇关于这种蛋白质的制备和生物化学研究的论文。 不同长度的wzz蛋白，这被认为是与不同的生物功能，将可用于SAXS研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Wzz protein is a chain length determinant protein involving in O-antigen component of lipopolysaccharide (LPS) biosynthesis, and also behaves as a polymerase by incorporated various length of repeated O-units (O-antigen), whose concept is correlated to our proposed studies for DNA polymerase. Various other biophysical methods (crystallography, NMR and Cryo-EM) have been attempted in the past years to obtain the structural information of this membrane protein wzz (Guo, H. et al. (2005) Appl. Environ. Microbiol., 71, 7995-8001). We have collaborated since three months ago with Prof. George Wang at the OSU regarding obtaining its structural information by SAXS. While no significant progress has been made by other methods, we have some very positive preliminary studies for this protein by SAXS. We would like use this rapid access for obtaining more preliminary SAXS studies before we submit our full proposal. Two papers regarding the preparation of this protein and biochemical studies have been published. Different length of wzz proteins, which are proposed to be correlated to different biological functions, will be available for SAXS studies.