SAXS STUDIES OF E COLI WZZ PROTEIN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 WZZ蛋白是一种链长决定蛋白，参与了脂多糖(LPS)生物合成中的O-抗原成分，同时也是一种聚合酶，它结合了不同长度的重复O-单位(O-抗原)，这一概念与我们提出的DNA聚合酶的研究相关联。在过去的几年里，人们尝试了各种其他的生物物理方法(结晶学、核磁共振和冷冻-EM)来获得这种膜蛋白WZZ的结构信息(Guo，H.et al.(2005)应用环境。微生物，71,7995-8001)。自三个月前以来，我们一直与俄亥俄州立大学的乔治·王教授合作，通过SAXS获取其结构信息。虽然其他方法没有取得重大进展，但我们已经通过SAXS对这种蛋白质进行了一些非常积极的初步研究。在我们提交完整的提案之前，我们希望使用这种快速访问来获得更多SAXS初步研究。关于这种蛋白质的制备和生化研究的两篇论文已经发表。不同长度的WZZ蛋白被认为与不同的生物学功能相关，将可用于SAXS研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Wzz protein is a chain length determinant protein involving in O-antigen component of lipopolysaccharide (LPS) biosynthesis, and also behaves as a polymerase by incorporated various length of repeated O-units (O-antigen), whose concept is correlated to our proposed studies for DNA polymerase. Various other biophysical methods (crystallography, NMR and Cryo-EM) have been attempted in the past years to obtain the structural information of this membrane protein wzz (Guo, H. et al. (2005) Appl. Environ. Microbiol., 71, 7995-8001). We have collaborated since three months ago with Prof. George Wang at the OSU regarding obtaining its structural information by SAXS. While no significant progress has been made by other methods, we have some very positive preliminary studies for this protein by SAXS. We would like use this rapid access for obtaining more preliminary SAXS studies before we submit our full proposal. Two papers regarding the preparation of this protein and biochemical studies have been published. Different length of wzz proteins, which are proposed to be correlated to different biological functions, will be available for SAXS studies.