EFFORTS TOWARD THE EXPANSION OF THE GENETIC ALPHABET

扩展遗传字母表的努力

• 批准号: 7598069
• 负责人: SHIGEO MATSUDA
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598069
• 关键词: Base Pairing; Benzofurans; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Structure; DNA-Directed DNA Polymerase; Fluorobenzenes; Funding; Genetic; Grant; Institution; Nucleic Acid Biochemistry; Object Attachment; Research; Research Personnel; Resources; Source; United States National Institutes of Health; analog; benzofuran; design; improved; insight; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We designed novel unnatural base pairs lacking Watson-Crick H-bonds and examined both their stability in DNA and their replication by DNA polymerases for an expanded genetic alphabet. We found that BFR and FB, which are benzofuran and fluorobenzene analogs, respectively, are good candidates of a third base pair. To understand and improve these unnatural base pairs, we are trying to solve the DNA structures. These studies are expected to provide unique insights into nucleic acid biochemistry.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们设计了新的非天然碱基对缺乏沃森-克里克H-键，并检查了它们在DNA中的稳定性和它们的复制DNA聚合酶的扩展的遗传字母表。我们发现，BFR和FB，这是苯并呋喃和氟苯类似物，分别是很好的候选人的第三个碱基对。为了理解和改进这些非自然碱基对，我们正试图解决DNA结构。这些研究有望为核酸生物化学提供独特的见解。