SPECTRAL ANALYSIS FLOW CYTOMETRY

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 一种能够进行光谱分析的流式细胞仪正在开发中。 目前，所有流式细胞仪都通过分色镜加光谱滤光片分离荧光发射。 本开发的目标是通过用线CCD阵列记录光谱分散光来分析荧光发射。 初步计算表明，背面变薄的CCD阵列具有足够的灵敏度来检测来自激光束中的细胞的光谱色散光100微秒。 已从Hamamatsu Corporation获得背面变薄的探测器评估套件。 它被集成到现有的流式细胞仪进行评估。 在测试结果出来之前，我们将购买CCD阵列永久集成到流量系统中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A spectral analysis capable flow cytometer is being developed. Currently, all flow cytometers separate fluorescence emissions by dichroic mirrors plus spectral filters. The goal of this development is to analyze the fluorescence emissions by recording with a line CCD array spectrally dispersed light. Preliminary calculations indicate that back thinned CCD arrays have sufficient sensitivity to detect spectrally dispersed light from cells that are in the laser beam for 100 microseconds. A back thinned detector evaluation kit has been obtained from Hamamatsu Corporation. It is being integrated into an existing flow cytometer for evaluation. Pending the outcome of the tests, we will purchase s CCD array for permanent integration into the flow system.