FLUORESCENCE LIFETIMES OF NADH IN GLYCEROL/WATER AND TREHALOSE
FLUORESCENCE LIFETIMES OF NADH IN GLYCEROL/WATER AND TREHALOSE
批准号:
7598458
负责人:
Jane Marie Vanderkooi
金额:
$0.16万
依托单位国家:
美国
项目类别:
财政年份:
2007
资助国家:
美国
项目状态:
已结题
起止时间:
2007-09-01 至 2008-05-31
关键词:
CellsComputer Retrieval of Information on Scientific Projects DatabaseDependenceFluorescenceFundingGlassGlycerolGrantImageInstitutionLocationOxidation-ReductionRangeRelaxationResearchResearch PersonnelResourcesSourceSucroseTemperatureTissuesTrehaloseUnited States National Institutes of HealthWatercold temperaturefluorescence imagingsugarwater solution
中文摘要
这个子项目是众多研究子项目之一
英文摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
Fluorescence imaging of cells and tissue can be used to evaluate beta-NADH redox and location. At low temperature beta-NADH fluorescence intensity is known to increase and therefore sensitivity of imaging increases. We have evaluated the temperature dependence of steady-state fluorescence for beta-NADH in glycerol/water solution and in trehalose/sucrose glass. The temperature range studied was 290 K to 12 K. The steady-state fluorescence of beta-NADH in glycerol/water was found to increase ~16 fold and the emission to shift by about 35 nm to the blue as temperature decreased. Much smaller changes were seen for fluorescence of beta-NADH in sugar glass. It is suggested that the sensitivity of beta-NADH fluorescence is related to water relaxation around the excited state molecule. We would like to probe further if the fluorescence lifetime of beta-NADH is dependent upon water concentration in glycerol/water mixtures.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
2D IR OF GUANIDINE IN WATER
-
批准号:8362573
-
项目类别:
-
资助金额:$1.95万
-
财政年份:2011
-
负责人:Jane Marie Vanderkooi
-
依托单位:
2D IR OF GUANIDINE IN WATER
-
批准号:8169549
-
项目类别:
-
资助金额:$4.99万
-
财政年份:2010
-
负责人:Jane Marie Vanderkooi
-
依托单位:
2D IR OF GUANIDINE IN WATER
-
批准号:7955455
-
项目类别:
-
资助金额:$2.88万
-
财政年份:2009
-
负责人:Jane Marie Vanderkooi
-
依托单位:
2D IR OF GUANIDINE IN WATER
-
批准号:7723866
-
项目类别:
-
资助金额:$1.55万
-
财政年份:2008
-
负责人:Jane Marie Vanderkooi
-
依托单位:
CORE--Equipment Core
-
批准号:7482997
-
项目类别:
-
资助金额:$4.58万
-
财政年份:2007
-
负责人:Jane Marie Vanderkooi
-
依托单位:
LIFETIME DECAYS OF ROSE BENGAL BOUND TO TRYPSIN
-
批准号:7183291
-
项目类别:
-
资助金额:$0.67万
-
财政年份:2005
-
负责人:Jane Marie Vanderkooi
-
依托单位:
Admin Core
-
批准号:7529249
-
项目类别:
-
资助金额:$1.96万
-
财政年份:2005
-
负责人:Jane Marie Vanderkooi
-
依托单位:
EFFECT OF TREHALOSE ON METAL FREE & ZN PORPHYRIN CYTOCHROME C
-
批准号:6976499
-
项目类别:
-
资助金额:$1.31万
-
财政年份:2004
-
负责人:Jane Marie Vanderkooi
-
依托单位:
MPHRP EMISSION SPECTROSCOPY
-
批准号:6976519
-
项目类别:
-
资助金额:$0.65万
-
财政年份:2004
-
负责人:Jane Marie Vanderkooi
-
依托单位:
TRYPTOPHAN LIFETIMES IN MELLITIN AND MELLITIN/CYCLODEXTRIN
-
批准号:6976524
-
项目类别:
-
资助金额:$0.65万
-
财政年份:2004
-
负责人:Jane Marie Vanderkooi
-
依托单位:
Admin Core
-
批准号:6707811
-
项目类别:
-
资助金额:$1.9万
-
财政年份:2003
-
负责人:Jane Marie Vanderkooi
-
依托单位:
Equip Core
-
批准号:6707814
-
项目类别:
-
资助金额:$8.53万
-
财政年份:2003
-
负责人:Jane Marie Vanderkooi
-
依托单位:
Vibrational and Optical Probes of Electric Fields in Proteins
-
批准号:6706154
-
项目类别:
-
资助金额:$11.21万
-
财政年份:2003
-
负责人:Jane Marie Vanderkooi
-
依托单位:
PROTON DYNAMICS IN FREE BASE PORPHYRINS IN EXCITED SINGLET STATE
-
批准号:6480831
-
项目类别:
-
资助金额:$15.58万
-
财政年份:2001
-
负责人:Jane Marie Vanderkooi
-
依托单位:
INFRARED HOLEBURNING IN PROTEINS
-
批准号:6480856
-
项目类别:
-
资助金额:$15.58万
-
财政年份:2001
-
负责人:Jane Marie Vanderkooi
-
依托单位:
ORIGIN OF SPECTRAL SPLITTING IN HEMOPROTEINS
-
批准号:6480830
-
项目类别:
-
资助金额:$15.58万
-
财政年份:2001
-
负责人:Jane Marie Vanderkooi
-
依托单位:
CORE--EQUIPMENT
-
批准号:6336547
-
项目类别:
-
资助金额:$14.76万
-
财政年份:2000
-
负责人:Jane Marie Vanderkooi
-
依托单位:
VIBRATIONAL AND OPTICAL PROBES OF ELECTRIC FIELDS
-
批准号:6336543
-
项目类别:
-
资助金额:$14.76万
-
财政年份:2000
-
负责人:Jane Marie Vanderkooi
-
依托单位:
PROTON DYNAMICS IN FREE BASE PORPHYRINS IN EXCITED SINGLET STATE
-
批准号:6328035
-
项目类别:
-
资助金额:$0.05万
-
财政年份:2000
-
负责人:Jane Marie Vanderkooi
-
依托单位:
INFRARED HOLEBURNING IN PROTEINS
-
批准号:6328060
-
项目类别:
-
资助金额:$0.27万
-
财政年份:2000
-
负责人:Jane Marie Vanderkooi
-
依托单位: