FLUORESCENCE LIFETIMES OF NADH IN GLYCEROL/WATER AND TREHALOSE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 细胞和组织的荧光成像可以用来评估β-NADH的氧化还原和定位。在低温下，已知β-NADH的荧光强度增加，因此成像的灵敏度增加。我们评估了β-NADH在甘油/水溶液和海藻糖/蔗糖玻璃中的稳态荧光的温度依赖性。在290K~12K的温度范围内，β-NADH在甘油/水中的稳态荧光随温度的降低而增加约16倍，发射向蓝移约35 nm。糖玻璃中β-NADH的荧光变化要小得多。结果表明，β-NADH荧光的灵敏度与激发态分子周围的水弛豫有关。我们想进一步探讨β-NADH的荧光寿命是否依赖于甘油/水混合物中的水浓度。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Fluorescence imaging of cells and tissue can be used to evaluate beta-NADH redox and location. At low temperature beta-NADH fluorescence intensity is known to increase and therefore sensitivity of imaging increases. We have evaluated the temperature dependence of steady-state fluorescence for beta-NADH in glycerol/water solution and in trehalose/sucrose glass. The temperature range studied was 290 K to 12 K. The steady-state fluorescence of beta-NADH in glycerol/water was found to increase ~16 fold and the emission to shift by about 35 nm to the blue as temperature decreased. Much smaller changes were seen for fluorescence of beta-NADH in sugar glass. It is suggested that the sensitivity of beta-NADH fluorescence is related to water relaxation around the excited state molecule. We would like to probe further if the fluorescence lifetime of beta-NADH is dependent upon water concentration in glycerol/water mixtures.