FLUORESCENCE LIFETIMES OF NADH IN GLYCEROL/WATER AND TREHALOSE

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细胞和组织的荧光成像可用于评估β-NADH氧化还原和定位。在低温下，已知β-NADH荧光强度增加，因此成像的灵敏度增加。我们已经评估了β-NADH在甘油/水溶液和海藻糖/蔗糖玻璃中的稳态荧光的温度依赖性。研究的温度范围为290 K至12 K。发现β-NADH在甘油/水中的稳态荧光增加约16倍，并且随着温度降低，发射向蓝色偏移约35 nm。糖玻璃中β-NADH的荧光变化要小得多。这表明β-NADH荧光的灵敏度与激发态分子周围的水弛豫有关。我们想进一步探索β-NADH的荧光寿命是否取决于甘油/水混合物中的水浓度。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Fluorescence imaging of cells and tissue can be used to evaluate beta-NADH redox and location. At low temperature beta-NADH fluorescence intensity is known to increase and therefore sensitivity of imaging increases. We have evaluated the temperature dependence of steady-state fluorescence for beta-NADH in glycerol/water solution and in trehalose/sucrose glass. The temperature range studied was 290 K to 12 K. The steady-state fluorescence of beta-NADH in glycerol/water was found to increase ~16 fold and the emission to shift by about 35 nm to the blue as temperature decreased. Much smaller changes were seen for fluorescence of beta-NADH in sugar glass. It is suggested that the sensitivity of beta-NADH fluorescence is related to water relaxation around the excited state molecule. We would like to probe further if the fluorescence lifetime of beta-NADH is dependent upon water concentration in glycerol/water mixtures.