INTERACTION BETWEEN BLASTOCYST AND UTERINE EPITHELIUM

囊胚和子宫上皮之间的相互作用

• 批准号: 7601305
• 负责人: R. MICHAEL ROBERTS
• 金额: $ 0.03万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601305
• 关键词: Achievement; Aspartic Endopeptidases; Binding; Biological; Cattle; Class; Classification; Complementary DNA; Computer Retrieval of Information on Scientific Projects Database; Conceptus; Embryo; Epithelium; Exhibits; Expressed Sequence Tags; Family; Funding; Gene Family; Genes; Glycoproteins; Grant; Immune; Institution; Interferon Type I; Interferon-alpha; Pepsin A; Phylogeny; Pregnancy; Publications; Rate; Relative (related person); Research; Research Personnel; Resources; Sequence Analysis; Series; Sheep; Source; Tissues; United States National Institutes of Health; base; blastocyst; comparative; day; implantation; interest; member; polypeptide; tau interferon; trophoblast

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sequence analysis of ESTs and cDNA from trophoblast tissues of cattle, sheep and relatives. We also have a particular interest in gene families that have trophoblast-specific expression and that are evolving at high rates. These families include the interferon-tau, which are Type I interferon (IFN) structurally related to the IFN-alpha. Their genes are not virally inducible; instead they are constitutively expressed for a few days prior to implantation. A second family is the pregnancy- associated glycoproteins (PAG), which are structurally related to pepsin and other aspartic proteinases. There are over 100 members and they appear to be a new class of polypeptide-binding molecules, possibly involved in immune protection of the embryo. The third family is also large and consists of a group of polypeptides with Kunitz domains at their amino termini, preceded by a series of hydrophobic repeats, which exhibit overall general similarity but are nevertheless distinct in sequence. Publications - Winkelman, GL, Roberts, RM, Peterson, AJ, Alexenko, AP, and Ealy, AD.: "Identification of the Expressed Forms of Ovine Interferon-Tau in the Periimplantation conceptus: Sequence Relationships and Comparative Biological Activities.": Biol. Reprod.: 61:1592-1600:1999. Hughes, AL, Green, JA, Garbayo JM, and Roberts, RM.: "Adaptive diversification within a large family of recently duplicated, placentally expressed genes.": Proc. Natl. Acad. Sci.: 97:3319-3323: 2000. Achievements - A classification of the interferon-tau based on strucural phylogeny.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 牛、羊及其近缘种滋养层组织EST和cDNAs的序列分析。我们还对具有滋养层细胞特异性表达的基因家族特别感兴趣，这些基因家族正在以高速率进化。这些家族包括干扰素-tau，它是结构上与干扰素-α相关的I型干扰素(干扰素)。它们的基因不能通过病毒诱导；相反，它们在植入前几天进行结构性表达。第二个家族是妊娠相关糖蛋白(PAG)，它在结构上与胃酶和其他天冬氨酸蛋白酶相关。有100多个成员，它们似乎是一类新的多肽结合分子，可能参与胚胎的免疫保护。第三个家族也很大，由一组多肽组成，在它们的氨基末端有kunitz域，前面有一系列疏水重复序列，它们总体上表现出总体上的相似性，但在序列上却是不同的。出版物--Winkelman，GL，Roberts，RM，Peterson，AJ，Alexenko，AP，和Ealy，AD.：“Ovine Interferon-Tau在围植入期胚胎中表达形式的鉴定：序列关系和比较生物学活动.”报告：61：1592-1600：1999。Hughes，AL，Green，JA，Garbayo JM，and Roberts，RM.：“最近复制的、位置上表达的基因大家族中的适应性多样化。”娜塔莉。阿卡德。科学编号：97：3319-3323：2000。成就-基于结构系统学对干扰素-tau的分类。