INTERACTION BETWEEN BLASTOCYST AND UTERINE EPITHELIUM

囊胚和子宫上皮之间的相互作用

• 批准号: 7601305
• 负责人: R. MICHAEL ROBERTS
• 金额: $ 0.03万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7601305
• 关键词: Achievement; Aspartic Endopeptidases; Binding; Biological; Cattle; Class; Classification; Complementary DNA; Computer Retrieval of Information on Scientific Projects Database; Conceptus; Embryo; Epithelium; Exhibits; Expressed Sequence Tags; Family; Funding; Gene Family; Genes; Glycoproteins; Grant; Immune; Institution; Interferon Type I; Interferon-alpha; Pepsin A; Phylogeny; Pregnancy; Publications; Rate; Relative (related person); Research; Research Personnel; Resources; Sequence Analysis; Series; Sheep; Source; Tissues; United States National Institutes of Health; base; blastocyst; comparative; day; implantation; interest; member; polypeptide; tau interferon; trophoblast

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sequence analysis of ESTs and cDNA from trophoblast tissues of cattle, sheep and relatives. We also have a particular interest in gene families that have trophoblast-specific expression and that are evolving at high rates. These families include the interferon-tau, which are Type I interferon (IFN) structurally related to the IFN-alpha. Their genes are not virally inducible; instead they are constitutively expressed for a few days prior to implantation. A second family is the pregnancy- associated glycoproteins (PAG), which are structurally related to pepsin and other aspartic proteinases. There are over 100 members and they appear to be a new class of polypeptide-binding molecules, possibly involved in immune protection of the embryo. The third family is also large and consists of a group of polypeptides with Kunitz domains at their amino termini, preceded by a series of hydrophobic repeats, which exhibit overall general similarity but are nevertheless distinct in sequence. Publications - Winkelman, GL, Roberts, RM, Peterson, AJ, Alexenko, AP, and Ealy, AD.: "Identification of the Expressed Forms of Ovine Interferon-Tau in the Periimplantation conceptus: Sequence Relationships and Comparative Biological Activities.": Biol. Reprod.: 61:1592-1600:1999. Hughes, AL, Green, JA, Garbayo JM, and Roberts, RM.: "Adaptive diversification within a large family of recently duplicated, placentally expressed genes.": Proc. Natl. Acad. Sci.: 97:3319-3323: 2000. Achievements - A classification of the interferon-tau based on strucural phylogeny.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 牛、羊及其近缘种滋养层组织EST和cDNA序列分析。我们还对具有滋养层特异性表达和高速进化的基因家族特别感兴趣。这些家族包括干扰素-tau，其是结构上与IFN-α相关的I型干扰素（IFN）。它们的基因不是病毒诱导的;相反，它们在植入前几天组成型表达。第二个家族是妊娠相关糖蛋白（PAG），其在结构上与胃蛋白酶和其他天冬氨酸蛋白酶相关。有超过100个成员，它们似乎是一类新的多肽结合分子，可能参与胚胎的免疫保护。第三个家族也很大，由一组在其氨基末端具有Kunitz结构域的多肽组成，前面是一系列疏水重复序列，其表现出总体上的相似性，但序列不同。 出版物- Winkelman，GL，Roberts，RM，Peterson，AJ，Alexenko，AP，and Ealy，AD.：“识别围植入期孕体中绵羊干扰素-Tau的表达形式：序列关系和比较生物学活性。“：生物学再现：61：1592-1600：1999。休斯，AL，绿色，JA，加尔巴约JM和罗伯茨，RM：“在一个最近复制的胎盘表达基因大家族中的适应性多样化。“：Proc. Natl. Acad.科学：97：3319-3323：2000。 成就-基于结构发生学的干扰素-tau的分类。