IN VIVO FRET ASSAY FOR DETECTING KINASE ACTIVATION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 酵母中原癌基因同系物Ras的激活导致蛋白激酶A（PKA）的激活。 目前还不存在直接监测PKA体内活化的测定。 我们建议测试是否在体内FRET检测方法的基础上开发的罗杰钱和他的同事用于哺乳动物细胞将在酵母中发挥作用。 FRET检测PKA活化的可用性将极大地促进酵母中营养信号的生理学研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Activation of the protooncogene homolog Ras in yeast results in activation of protein kinase A (PKA). No assay currently exists to directly monitor activation of PKA in vivo. We propose to test whether an in vivo FRET assay based on a method developed by Roger Tsien and his colleagues for use in mammalian cells will function in yeast. The availability of a FRET assay for PKA activation would greatly facilitate physiological studies on nutrient signaling in yeast.