IN VIVO FRET ASSAY FOR DETECTING KINASE ACTIVATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 酵母中原癌基因Ras的激活导致蛋白激酶A(PKA)的激活。目前还没有一种方法可以直接监测体内PKA的激活。我们建议测试基于Roger Tsien和他的同事开发的用于哺乳动物细胞的体内FRET测试是否会在酵母中发挥作用。PKA活性的FRET检测方法的建立将极大地促进酵母中营养信号的生理学研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Activation of the protooncogene homolog Ras in yeast results in activation of protein kinase A (PKA). No assay currently exists to directly monitor activation of PKA in vivo. We propose to test whether an in vivo FRET assay based on a method developed by Roger Tsien and his colleagues for use in mammalian cells will function in yeast. The availability of a FRET assay for PKA activation would greatly facilitate physiological studies on nutrient signaling in yeast.