BIOSYNTHETIC MECHANISMS FOR FUNGAL POLYKETIDES

真菌聚酮的生物合成机制

• 批准号: 7610436
• 负责人: LIANGCHENG DU
• 金额: $ 2.86万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610436
• 关键词: Aspergillus; Biochemical Genetics; Biological Factors; Cholesterol; Complement; Complex; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Escherichia coli; Fumonisins; Funding; Fusarium; Genes; Grant; Institution; Knock-out; Lovastatin; Molds; Mycotoxins; Pharmaceutical Preparations; Reaction; Research; Research Personnel; Resources; Role; Saccharomyces cerevisiae; Source; United States National Institutes of Health; fungus

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many important polyketide natural products are produced by filamentous fungi, such as the mycotoxin fumonisins produced by Fusarium verticillioides and the cholesterol-lowering drug lovastatin (MervacorTM) by Aspergillus terreus. We are using genetic and biochemical approaches to understand the mechanism by which the fungi synthesize these complex metabolites. The biosynthetic genes are specifically knocked out and functionally complemented to define the role of the genes. The biosynthetic enzymes are produced in heterologous hosts, such as E. coli and bakers yeast, to determine the reactions catalyzed by these enzymes.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 许多重要的聚酮化合物天然产物是由丝状真菌产生的，例如由轮枝镰刀菌（Fusarium verticillioides）产生的真菌毒素伏马菌素（fumonisins）和由土曲霉（Aspergillus terreus）产生的降胆固醇药物洛伐他汀（MervacorTM）。我们正在使用遗传和生物化学方法来了解真菌合成这些复杂代谢物的机制。生物合成基因被特异性敲除并在功能上互补以定义基因的作用。生物合成酶在异源宿主中产生，例如E.大肠杆菌和面包师的酵母，以确定这些酶催化的反应。