MOUSE TRANSGENIC & KNOCKOUT CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 转基因小鼠和基因敲除核心为布朗、附属医院和地区研究人员创造了转基因动物。这一设施包括一个独立的显微注射设施，能够处理亲核显微注射和囊胚微注射。一个ES细胞设备附在核心上；它的功能是用研究人员提供的载体执行基因靶向操作。该机构还就构建载体的最佳策略向调查人员提供建议。除了载体构建，核心处理转基因动物生产的方方面面。该中心还就后续的育种方案和转基因动物的分析向研究人员提供建议。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Transgenic Mouse and Knockout Core creates transgenic animals for Brown, affiliated hospital, and re-gional investigators. This facility includes a stand-alone microinjection facility capable of handling both pro-nuclear and blastocyst microinjections. An ES cell facility is attached to the core; its function is to perform gene targeting manipulations with vectors supplied by investigators. The facility also advises investigators on optimal strategies for the construction of vectors. With the exception of vector construction, the core handles all aspects of the production of transgenic animals. The core also advises investigators on follow up breeding regimens and analysis of the transgenic animals.