MOUSE TRANSGENIC & KNOCKOUT CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 转基因小鼠和敲除核心为布朗，附属医院和地区研究人员创造转基因动物。该设施包括一个独立的显微注射设施，能够处理促核和囊胚显微注射。一个ES细胞设施连接到核心;其功能是用研究者提供的载体进行基因靶向操作。该设施还就构建载体的最佳策略向研究人员提供咨询。除载体构建外，核心处理转基因动物生产的所有方面。该中心还就转基因动物的后续育种方案和分析向研究人员提供建议。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Transgenic Mouse and Knockout Core creates transgenic animals for Brown, affiliated hospital, and re-gional investigators. This facility includes a stand-alone microinjection facility capable of handling both pro-nuclear and blastocyst microinjections. An ES cell facility is attached to the core; its function is to perform gene targeting manipulations with vectors supplied by investigators. The facility also advises investigators on optimal strategies for the construction of vectors. With the exception of vector construction, the core handles all aspects of the production of transgenic animals. The core also advises investigators on follow up breeding regimens and analysis of the transgenic animals.