MICROCHEMISTRY and PROTEOMICS

显微化学和蛋白质组学

• 批准号: 7671830
• 负责人: PAUL TEMPST
• 金额: $ 20.2万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7671830
• 关键词: Acetylation; Affinity; Affinity Chromatography; Alanine; Amino Acid Sequence; Amino Acids; Anti-Bacterial Agents; Antibodies; Area; Arginine; Base Sequence; Binding; Biological; Biological Process; Biomedical Research; Biotinylation; Cell Extracts; Cells; Chemicals; Chemistry; Classification; Clinical Research; Companions; Complex; Coupling; DNA Repair; Development; Disease; Dissection; Exhibits; Facility Construction Funding Category; Generations; Glutamine; Glycine; Goals; Growth; High Pressure Liquid Chromatography; Immunologics; Isotopes; Label; Laboratories; Length; Ligands; MALDI-TOF Mass Spectrometry; Manuals; Mass Spectrum Analysis; Measurement; Membrane; Memorial Sloan-Kettering Cancer Center; Methods; Methylation; Microchemistry; Modeling; Modification; Molecular; Monoclonal Antibodies; Mutation; N-terminal; Nature; Normal tissue morphology; Nucleic acid sequencing; Numbers; Pattern; Peptide Sequence Determination; Peptide Synthesis; Peptide Vaccines; Peptides; Phase; Phosphorylation; Positioning Attribute; Post Translational Modification Analysis; Post-Translational Protein Processing; Process; Protein Analysis; Protein Sequence Analysis; Proteins; Proteomics; Purpose; Quality Control; Radio; Radiolabeled; Reagent; Relative (related person); Reporting; Research; Research Personnel; Research Priority; Research Project Grants; Retrieval; Sampling; Scanning; Screening procedure; Services; Signal Pathway; Specific qualifier value; Specificity; Stereoisomer; Structure; Surface; Techniques; Vaccines; Weight; analog; base; cancer cell; cell growth; crosslink; disulfide bond; enantiomer; glycosylation; immunogenic; inhibitor/antagonist; instrument; instrumentation; molecular mass; polypeptide; programs; protein protein interaction; radiotracer; research study; response; senescence; sulfation; synthetic peptide

The Microchemistry and Proteomics Facility (MPF) supports laboratory and clinical research programs at MSKCC by (i) providing synthetic peptides and by (ii) performing mass spectrometric and amino acid sequence analyses of proteins and peptides, generally for the purpose of identification, relative quantitative analysis and post-translational modification (PTM) analysis. Synthetic molecules are made according to specifications provided by the investigator. Samples submitted for identification (either single proteins or mixtures), for PTM analysis or N-terminal sequencing are prepared by the lab requesting service. Information on chemistries and instrumentation, and expert advice on experimental approaches are provided by facility staff when needed. The goal of proteomics is to analyze changing levels, local concentrations and post-translational modifications of proteins in a cell, as well as to analyze higher-order networks of protein-protein interactions. Proteins and protein modifications are critical in the complex signaling pathways and regulatory processes underlying cell growth, division, differentiation, DNA repair, development, senescence, and in responses to bioactive agents, genetic alterations and disease. Dissection of multi-component protein complexes is therefore increasingly the focus of studies on molecular control mechanisms. Identification of unique components provides a powerful approach to the selective retrieval and identification of the companions. The impact that these services have provided, in facilitating research projects and enabling the timely progression of high priority research, can be recognized throughout the reports of the several research programs.

微量化学和蛋白质组学设施（MPF）支持实验室和临床研究计划， MSKCC通过（i）提供合成肽和通过（ii）进行质谱和氨基酸分析， 蛋白质和肽的序列分析，一般用于鉴定，相对定量 分析和翻译后修饰（PTM）分析。合成分子是根据 研究者提供的质量标准。提交用于鉴定的样品（单一蛋白质或 混合物），用于PTM分析或N-末端测序，由请求服务的实验室制备。 提供了有关化学和仪器的信息，以及有关实验方法的专家建议 必要时由设施工作人员提供。 蛋白质组学的目标是分析变化的水平，局部浓度和翻译后 细胞中蛋白质的修饰，以及分析蛋白质-蛋白质相互作用的高阶网络。 蛋白质和蛋白质修饰在复杂的信号通路和调控过程中至关重要 潜在的细胞生长、分裂、分化、DNA修复、发育、衰老，以及对 生物活性剂、遗传改变和疾病。多组分蛋白质复合物的解剖是 因此，越来越多的研究的重点是分子控制机制。唯一标识 组件提供了一个强大的方法来选择性检索和识别的同伴。的 这些服务在促进研究项目和及时提供 高优先级研究的进展，可以在几项研究的报告中得到承认 程序.