CORE 1 TRP2: PROTEASE ACTIVITY IMAGING TECHNOLOGY (PAIT)

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 技术研究项目2，蛋白酶活性成像技术（PAIT），将开发 三维和四维细胞蛋白水解成像技术。两个独立且 将采取补充办法。首先，高通量底物噬菌体展示系统将 设计用于鉴定绝大多数编码的蛋白酶的高度选择性指示底物 在基因组中。第二，将应用化学策略来开发基于活性的探针， 酶促激活的荧光报告基因。这两套信标将在生化和 细胞模型，并将最终用于图像蛋白水解在细胞水平

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Technical Research Project 2, Protease Activity Imaging Technology (PAIT), will develop technology for imaging of cellular proteolysis in three and four dimensions. Two independent and complementary approaches will be taken. First, a high throughput substrate phage display system will be devised to identify highly selective indicator substrates for the vast majority of proteases encoded in the genome. Second, a chemical strategy will be applied to develop activity-based probes with an enzymatically activated fluorescent reporter. Both sets of beacons will be tested in biochemical and cellular models and will ultimately be used to image proteolysis at the cellular level