CORE 1 TRP2: PROTEASE ACTIVITY IMAGING TECHNOLOGY (PAIT)

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Technical Research Project 2, Protease Activity Imaging Technology (PAIT), will develop technology for imaging of cellular proteolysis in three and four dimensions. Two independent and complementary approaches will be taken. First, a high throughput substrate phage display system will be devised to identify highly selective indicator substrates for the vast majority of proteases encoded in the genome. Second, a chemical strategy will be applied to develop activity-based probes with an enzymatically activated fluorescent reporter. Both sets of beacons will be tested in biochemical and cellular models and will ultimately be used to image proteolysis at the cellular level