THE EFFECT OF ISOFLURANE ON TRANSCAPILLARY FLUID REDISTRIBUTION AFTER HEMORRHAG

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 液体治疗很重要，特别是在重病患者中，对于补充手术期间丢失的液体和其他元素也很重要。以前的绵羊研究表明，麻醉气体会促进血管外液体的积聚。此外，在绵羊模型中，麻醉气体已被证明会减弱毛细血管通常对液体流失的再充盈反应。 该项目的目标是确定在受控出血期间，麻醉气体是否减少尿液的产生并导致液体分配到人类参与者的血管外。为了实现这些目标，我们将招募12名志愿者参加我们的流体动力学研究。 将使用称为指示剂稀释技术的常用方法计算体液变化，以评估基线容量状态。测量基线后，将定期收集血红蛋白值。 每名参与者将在6个月内参加4组研究。 研究的4个组的描述如下： 1. 清醒，抽血，无液体输注（仅清醒） 2. 麻醉，抽血，无输液（仅睡眠） 3. 清醒、抽血、输液（清醒加输液） 4. 麻醉、抽血、输液（睡眠加输液） 本研究的重要性在于能够更好地管理常见临床事件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Fluid therapy is important, especially in very sick patients, and also important for the replacement of fluids and other elements lost during surgery. Previous sheep studies have shown that anesthesia gases promote the accumulation of fluid outside of blood vessels. Furthermore, in sheep models, anesthesia gases have been shown to blunt the refilling response that capillaries normally have to fluid loss. The goal of this project is to determine if anesthesia gases decrease the production of urine and cause fluid to be allocated outside the blood vessels in human participants during a controlled bleed. To accomplish these goals, we will enroll 12 volunteers into our fluid kinetics study. Body fluid shifts will be calculated using a commonly used method known as the indicator dilution technique to asses the baseline volume status. After the baseline is measured, hemoglobin values will be collected at regular intervals. Each participant will take part in 4 arms of the study over a period of six months. The description of the 4 arms of the study is as follows: 1. conscious, blood withdrawal, no fluid infusion (awake only) 2. anesthetized, blood withdrawal, no fluid infusion (asleep only) 3. conscious, blood withdrawal, fluid infusion (awake plus infusion) 4. anesthetized, blood withdrawal, fluid infusion (asleep plus infusion) The importance of this study is the ability to better manage a common clinical occurrence.