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Characterization of the Sigma-2 receptor

Sigma-2 受体的表征

基本信息

批准号：
7712217
负责人：
Arnold Eino Ruoho
金额：
$ 18.56万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-08-01 至 2011-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7712217
关键词：
Affinity Affinity Chromatography Alkynes Amino Acid Sequence Antineoplastic Agents Applications Grants Avidin Azides Binding Binding Proteins Biochemical Biology Biotin Biotinylation Blast Cell Cell physiology Characteristics Chemicals Chemistry Cloning Complementary DNA Computer Simulation Copper DNA Sequence Data Databases Detergents Digestion Drug Addiction Drug abuse Event Family Figs - dietary Gel Genetic Goals Growth Ligand Binding Ligands Literature Liver Mass Spectrum Analysis Membrane Mental disorders Methods Molecular Biology Neuraxis Neuroglia Neurons Normal Cell Organ Peripheral Pharmaceutical Preparations Phenotype Photoaffinity Labels Piperazines Play Property Proteins Rattus Regulation Reporting Research Role Scheme Screening procedure Small Interfering RNA Structure Testing Therapeutic Tissues Validation base cell growth drug of abuse genome database innovation neoplastic cell public health relevance receptor receptor binding sigma receptors sigma-1 receptor sigma-2 receptor therapeutic target tool

项目摘要

DESCRIPTION (provided by applicant): The Sigma-2 receptor is a unique protein found in most mammalian tissues. Sigma-2 receptors play an important role in growth regulation in normal cells and tumor cells of neuronal and non neuronal origin. The Sigma-2 receptor has not been purified or cloned. This grant proposal is directed at purification and cloning of the Sigma-2 receptor. This goal will be accomplished through two alternate and complementary approaches. Specific Aim 1 is entitled: Purification, Identification and Cloning of the Sigma-2 receptor using a "Click Chemistry" approach. Specific Aim 2 is entitled: Identification and cloning of the Sigma-2 receptor using an In Silico approach. Characterization of the Sigma-2 receptor is the initial step leading to a basic understanding of the role of the Sigma-2 receptor in regulation of neuronal and non-neuronal cell function and for potential therapeutic strategies to treat drugs of abuse and/or aberrant cell growth. . PUBLIC HEALTH RELEVANCE: The sigma-2 receptor is a mammalian protein in the central nervous system and in peripheral organs that may be involved in drug addiction. Sigma-2 receptors play an important role in growth regulation in normal cells and in tumor cells of different origins. Despite the presence of numerous molecular biology tools, the sigma-2 receptor has managed to evade all cloning attempts to date. This project is directed at purification and identification of the sigma-2 receptor.

描述（由申请人提供）：Sigma-2受体是一种在大多数哺乳动物组织中发现的独特蛋白质。Sigma-2受体在神经和非神经来源的正常细胞和肿瘤细胞的生长调节中发挥重要作用。Sigma-2受体尚未被纯化或克隆。这项拨款提案是针对Sigma-2受体的纯化和克隆。这一目标将通过两种交替和互补的方法来实现。具体目标1的标题是：使用“点击化学”方法纯化、鉴定和克隆Sigma-2受体。具体目标2的标题是：利用芯片方法鉴定和克隆Sigma-2受体。Sigma-2受体的表征是基本理解Sigma-2受体在调节神经元和非神经元细胞功能中的作用的第一步，也是治疗药物滥用和/或异常细胞生长的潜在治疗策略的第一步。公共卫生相关性：sigma-2受体是中枢神经系统和外周器官中的一种哺乳动物蛋白，可能与药物成瘾有关。Sigma-2受体在正常细胞和不同来源的肿瘤细胞的生长调节中发挥重要作用。尽管存在许多分子生物学工具，sigma-2受体还是成功地躲过了迄今为止所有的克隆尝试。本项目旨在纯化和鉴定sigma-2受体。