STRATEGIES TO EXTEND PLATELET STORAGE

延长血小板储存的策略

• 批准号: 7689739
• 负责人: Sherrill J. Slichter
• 金额: $ 42.18万
• 依托单位: BLOODWORKS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7689739
• 关键词: Affect; Apoptosis; Autologous; Bacteria; Biological Assay; Bleeding time procedure; Blood; Blood Component Removal; Blood Platelets; Caring; Collection; Count; Data; Detection; Devices; Documentation; Ensure; Erythrocytes; Feces; Flow Cytometry; Grant; Hemorrhage; Hemostatic function; In Vitro; Individual; Label; Licensing; Measurement; Measures; Metabolism; Methods; Monitor; Monoclonal Antibodies; Patients; Plasma; Pre-Par; Procedures; Process; Radiolabeled; Rate; Recovery; Recruitment Activity; Relative (related person); Resources; Sodium Acetate; Solutions; Sterile coverings; Stretching; Suspension substance; Suspensions; Techniques; Temperature; Testing; Time; Transfusion; Whole Blood; bacterial resistance; base; coated pit; day; in vivo; pathogen; radiotracer; response; time use; volunteer

The primary objective of these studies is to determine whether platelets (pits) can be stored for longer than the currently-licensed 5 days. Furthermore, does the duration of pit storage depend on the type of pit product being stored (apheresis pits, pit concentrates prepared from pit-rich plasma (PRP), or from buffy coats (BC), pre-storage pooled PRP pit concentrates, or pathogen-reduced apheresis or BC pits), and the medium used for storage; i.e., plasma or a storage solution. The four critical questions that will be ad- dressed in our studies are: 1) does the method of pit collection using apheresis procedures versus prepar- ing pit concentrates from whole blood influence storage duration?; 2) does pre-storage pooling of pit con- centrates affect pit viability?; 3) do pit storage solutions allow pits to be stored longer than pits stored in plasma?; and 4) can pathogen-reduced pits be stored for extended time periods similar to non-pathogen reduced pits? Although in vitro tests will be performed to document post-storage pit counts as well as a va- riety of assays to determine post-storage pit function, metabolism and apoptosis, the post-storage quality of the extended stored pits will be based on in vivo measurements in normal volunteers and thrombocytopenic patients. Specifically, radiolabeled pit recovery and survival measurements of extended stored autologous pits in normal volunteers will be used to determine post-storage pit viability for all types of pit products stored in plasma versus a storage solution. For pits that are stored for longer than 8 days and/or are stored in a storage solution, transfusion studies in the thrombocytopenic patients will be used to evaluate pit viabil- ity by measuring the radiolabeled recovery and survival of the donors' pits following transfusion. Alterna- tively, patient responses to transfused donor pits will be determined by measuring post-transfusion pit in- crements, corrected count increments, and days-to-next transfusion. Pit function (i.e., hemostasis) following the transfusion of extended stored donor pits will be monitored by pit count versus bleeding time measure- ments and by radiochromium-labeled stool blood loss studies. At the conclusion of these studies, we should know how long each type of pits can be stored in plasma or Plasmalyte, the relative merits of each type of pits, and whether the extended stored pits are both viable and functional when given to thrombocytopenic patients.

这些研究的主要目的是确定血小板（坑）是否可以保存更长时间 比目前许可的 5 天。此外，坑存放的时间是否取决于坑的类型？ 储存的产品（单采坑、由富坑血浆 (PRP) 或血沉棕黄制备的坑浓缩物 外套 (BC)、预储存混合 PRP 坑浓缩液、或减少病原体的单采或 BC 坑），以及 用于存储的介质；即血浆或储存溶液。将提出的四个关键问题 我们的研究中涉及：1）使用单采术程序进行坑采集的方法与制备方法相比是否相同？ 从全血中提取的浓缩物会影响储存时间吗？ 2）是否预存池坑con- 浓缩液会影响坑的活力吗？ 3) 坑存储解决方案是否允许坑的存储时间比存储的坑更长 等离子体？; 4) 减少病原体的果核是否可以像非病原体一样延长储存时间 坑减少了？尽管将进行体外测试来记录储存后的坑计数以及 va- 通过一系列的测定来确定贮藏后坑的功能、代谢和细胞凋亡、贮藏后的质量 扩展储存坑将基于正常志愿者和血小板减少症患者的体内测量 患者。具体来说，放射性标记的坑恢复和长期保存的自体组织的存活测量 正常志愿者的果核将用于确定所有类型果核产品的储存后果核活力 储存在血浆中与储存溶液中。对于储存时间超过 8 天和/或储存的果核 在储存溶液中，血小板减少症患者的输血研究将用于评估坑生存能力 通过测量输血后供体坑的放射性标记恢复和存活来确定性。艾特纳- 积极地，患者对输血供体坑的反应将通过测量输血后坑内的情况来确定。 增量、校正计数增量以及距下次输血的天数。坑功能（即止血）如下 长期保存的供体坑的输血将通过坑计数与出血时间测量来监测- 和放射性铬标记的粪便失血研究。 在这些研究结束时，我们应该知道每种类型的凹坑可以在血浆中保存多长时间 或 Plasmalyte，每种类型坑的相对优点，以及扩展存储的坑是否都可行 给予血小板减少症患者时具有功能性。