LIGHT-INDUCED DNA BASE EXCITATION, DEACTIVATION AND OXIDATION

光诱导 DNA 碱基激发、失活和氧化

• 批准号: 7959219
• 负责人: Ruomei Gao
• 金额: $ 12.8万
• 依托单位: JACKSON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959219
• 关键词: Cells; Chloroform; Computer Retrieval of Information on Scientific Projects Database; DNA; Environmental Health; Funding; Grant; Institution; Lasers; Length; Light; Methods; Photons; Physiologic pulse; Production; Reaction; Research; Research Personnel; Resources; Singlet Oxygen; Sodium Chloride; Solutions; Source; System; Testing; United States National Institutes of Health; absorption; base; oxidation; phthalocyanine; triplet state; two-photon

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. An absolute actinometer is being developed for quantitative characterization of singlet oxygen production upon one- and two-photon excitation for a specific system, e.g., nanosensitizer, DNA bases, etc. Photochemical reactions were carried out under pulsed laser at 532 nm and 355 nm in a cylindrical cell of 5 cm light path length for obtaining efficient photon absorption and 1O2 trapping using DPBF as a singlet oxygen acceptor in highly diluted solutions. A well-established Reincke's salt actinometer was used for the quantitative determination of incident photons at both 532 and 355 nm. FD were determined in chloroform solutions with satisfied accuracy and precision for some phthalocyanines. These compounds were used as standards for testing this method. Key factors that influence 1O2 production through the competitions for both triplet states and singlet oxygen were explored.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 正在开发一种绝对日射计，用于定量表征特定系统在单光子和双光子激发下的单线态氧产生，例如，在532 nm和355 nm的脉冲激光下，在5 cm光程长的圆柱形池中进行光化学反应，以获得有效的光子吸收和1 O2捕获，使用DPBF作为高度稀释溶液中的单线态氧受体。一个完善的Reincke盐光辐射计用于定量测定入射光子在532和355 nm。在氯仿溶液中测定了某些酞菁类化合物的荧光强度，准确度和精密度均令人满意。这些化合物用作测试该方法的标准品。探讨了通过竞争三重态和单线态氧来影响1 O2产生的关键因素。