Translational Methods/Facilities Core (TMF - Core) (8 of 8)

转化方法/设施 (TMF) 核心将提供最先进的遗传分析以及 联盟所有组成部分的小鼠模型开发、表征和评估 神经精神表型组学。三个不同但互动的单位在操作上定义了具体目标 TMF 核心： ¿ 遗传研究单位将负责生成和分析所有遗传数据 联盟将进行多项关联研究。 ¿ BAG 转基因单位将使用 BAG 转基因或通过杂交创建新型小鼠模型 建立突变系 a) 通过遗传测试基因型/表型关联的细胞病因学 救援策略，b) 驱动荧光蛋白的细胞特异性表达，以识别中枢神经系统回路 可能会受到突变基因的干扰，并且 c) 对已识别的候选基因的贡献进行建模 和/或从正在进行的遗传关联分析中预期显着改变记忆 过程或反应抑制。 ¿ 啮齿动物表型分析单位与人类翻译应用核心密切合作 将设计、验证和进行行为分析，以对所使用的小鼠模型进行表型分析 财团。 这三个单位利用了加州大学洛杉矶分校大量的物质和智力资源。这种杠杆作用将 使 TMF-Core 能够经济高效地提供信息遗传/表型数据库， 在整个联盟中共享动物模型和经过验证的行为评估，随后 加州大学洛杉矶分校以外的研究界。

The Translational Methods/Facilities (TMF) Core will provide state of the art genetic analysis as well as mouse model development, characterization and assessment for all components of the Consortium for Neuropsychiatric Phenomics. Three distinct but interactive units operationally define the specific aims of the TMF-Core: ¿ The Genetic Studies Unit will be responsible for generating and analyzing all genetic data in the multiple association studies to be conducted by the Consortium. ¿ The BAG Transgenic Unit will create novel murine models using BAG transgenes or by crossing established mutant lines to a) test the cellular etiology of genotype/phenotype associations by genetic rescue strategies, b) drive cell-specific expression of fluorescent proteins to identify CNS circuitry that may be perturbed via mutant genes and c) model the contribution of candidate genes identified and/or anticipated from ongoing genetic association analysis to significantly modify memory processes or response inhibition. ¿ The Rodent Phenotyping Unit in close collaboration with the Human Translational Applications Core will design, validate and conduct behavioral assays to phenotype mouse models used by the Consortium. The three units leverage considerable physical and intellectual resources at UCLA. Such leveraging will enable the TMF-Core to cost-effectively and efficiently provide informational genetic/phenotype databases, shared animal models and validated behavioral assessments throughout the Consortium and subsequently to the research community outside of UCLA.