FUNCTIONAL ANALYSIS OF COMPLEMENT RECEPTOR 2 AS A LUPUS SUSCEPTIBILITY GENE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 补体受体2是抗体产生细胞上的一种蛋白质，被认为在狼疮中很重要。 我们已经证明，这种蛋白质的基因在狼疮动物模型中是异常的，这为它导致狼疮提供了强有力的证据。 我们还表明，这种蛋白质的基因中的某些变异在狼疮患者中更常见。 在这项研究中，我们希望确定补体受体2基因的变异如何影响其功能，以便我们能够更好地了解它如何参与狼疮。 为了做到这一点，我们将从表达不同版本基因的不同人身上抽取血液，以多种不同的方式测试受体的功能。 此外，我们将从一些人身上获得一小块扁桃体，以测试在扁桃体中表达但不在血液中表达的受体形式的功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Complement receptor 2 is a protein on antibody-producing cells believed to be important in lupus. We have shown that the gene for this protein is abnormal in an animal model of lupus, providing strong proof that it causes lupus. We have also shown that certain variations in the gene for this protein are found more often in individuals who have lupus. In this study, we want to determine how the variations in the complement receptor 2 gene affect its function so that we can understand better how it might be involved in lupus. To do this, we will draw blood from different people who express different versions of the gene to test the function of the receptor in a number of different ways. In addition, we will obtain a small piece of the tonsils from some people to test the function of a form of the receptor that is expressed in the tonsils but not in the blood.