INTRAMUSCULAR TRIGLYCERIDE TURNOVER AND INSULIN SENSITIVITY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的总体目标是阐明肌内甘油三酯（IMTG）周转和胰岛素敏感性之间的关系，在休息，运动和恢复。 我们建议研究胰岛素敏感（耐力训练的运动员）和不敏感的人（II型糖尿病患者）。 我们的目的是更好地理解高浓度IMTG与II型糖尿病的胰岛素敏感性呈负相关，但与耐力运动员的胰岛素敏感性呈正相关的悖论。这项研究还将解决性别之间IMTG利用的潜在差异，并确定IMTG是否是受过训练的个体在运动过程中的重要燃料来源。 我们假设IMTG营业额和利用率将增加运动员相比，II型糖尿病患者在休息，期间和运动后。我们假设增加IMTG营业额保护对减少胰岛素作用通常观察到的增加IMTG商店在II型糖尿病患者。 为了评估我们的假设，我们将招募受过耐力训练的运动员或II型糖尿病患者。胰岛素作用将首先通过频繁采样的静脉内葡萄糖耐量试验确定。 然后，受试者将进行一项代谢研究，在休息期间输注棕榈酸盐的稳定同位素，并在50% VO 2 max下运动1.5小时，然后在2小时恢复期间进行肌肉活检。然后使用示踪剂掺入IMTG池的分数掺入率计算IMTG转换。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our overall objective is to elucidate the relationship between intramuscular triglyceride (IMTG) turnover and insulin sensitivity during rest, exercise, and recovery. We are proposing to study insulin sensitive (endurance trained athletes) and insensitive individuals (Type II diabetics). We aim to better understand the paradox where high concentrations of IMTG are negatively associated with insulin sensitivity in Type II diabetes, yet positively associated with insulin sensitivity in endurance athletes. This study will also address potential differences in IMTG utilization between genders, and determine if IMTG is an important fuel source during exercise in trained individuals. We hypothesize IMTG turnover and utilization will be increased in athletes compared to Type II diabetics at rest, during, and after exercise. We postulate that increased IMTG turnover protects against the decreased insulin action commonly observed with increased IMTG stores in Type II diabetics. To evaluate our hypothesis we will recruit men and women who are either endurance trained athletes or Type II diabetics. Insulin action will first be determined via a frequently sampled intravenous glucose tolerance test. Subjects will then perform one metabolic study with an infusion of a stable isotope of palmitate and muscle biopsies during rest, 1.5 hours of exercise at 50% VO2max, and then during 2 hours of recovery. IMTG turnover will then be calculated using fractional incorporation rates of tracer into the IMTG pool.