PROTEIN PRODUCTION AND BIOPHYSICAL CHARACTERIZATION CORE

蛋白质生产和生物物理表征核心

• 批准号: 8364942
• 负责人: ANNE SKAJA ROBINSON
• 金额: $ 29.41万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364942
• 关键词: Beryllium; Centers of Research Excellence; Complex; Crystallization; Delaware; Detergents; Ensure; Funding; Grant; Light; Membrane Proteins; Methods; Names; National Center for Research Resources; Neutrons; Principal Investigator; Production; Proteins; Research; Research Infrastructure; Research Personnel; Resources; Roentgen Rays; Science; Solutions; Source; System; U-Series Cooperative Agreements; United States National Institutes of Health; Universities; cost; instrumentation; protein expression; reconstitution

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Protein Production and Biophysical Characterization Core encompasses the production component in the name of the COBRE as well as aspects of characterization. Specifically, the Core covers membrane protein expression, extraction, purification and, if necessary, reconstitution, as well as sufficient characterization to ensure their suitability for more detailed structural and functional analysis. Some research on protein-detergent complexes and their interactions, leading to crystallization, is part of the biophysical characterization effort as well. The Core provides instrumentation as well as scientific support, with a new element being a facility to assist investigators in developing and optimizing expression systems for proteins. A particular component of the characterization effort is devoted to scattering methods, of which it is especially X-ray and neutron scattering that represent distinctive and powerful capabilities that can shed considerable light on membrane proteins in solution. The COBRE is collaborating on neutron scattering methods with the Center for Neutron Science at the University of Delaware, which operates under a cooperative agreement with the National Center for Neutron Science.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 蛋白质生产和生物物理表征核心包括以COBRE名义的生产组件以及表征方面。具体而言，核心涵盖了膜蛋白表达，提取，纯化和（如有必要）重建，以及充分的表征，以确保其适用于更详细的结构和功能分析。对蛋白质-去污剂复合物及其相互作用（导致结晶）的一些研究也是生物物理表征工作的一部分。核心提供仪器和科学支持，其中一个新的元素是帮助研究人员开发和优化蛋白质表达系统的设施。表征工作的一个特定组成部分是致力于散射方法，其中特别是X射线和中子散射，代表了独特而强大的能力，可以在溶液中的膜蛋白上显示出相当大的光。COBRE正在与特拉华州大学的中子科学中心合作研究中子散射方法，该中心根据与国家中子科学中心的合作协议运作。