PROTEIN NMR SPECTROSCOPY CORE FACILITY

蛋白质核磁共振波谱核心设施

• 批准号: 8364929
• 负责人: JAMES F HINTON
• 金额: $ 15.01万
• 依托单位: UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364929
• 关键词: Applications Grants; Centers of Research Excellence; Collaborations; Commit; Consultations; Core Facility; Electrical Engineering; Funding; Grant; NMR Spectroscopy; National Center for Research Resources; Peptides; Principal Investigator; Protein NMR Spectroscopy; Proteins; Research; Research Infrastructure; Research Personnel; Resources; Solutions; Source; Training; United States National Institutes of Health; Universities; Wages; Writing; cost; experience; protein structure; protein structure function; solid state nuclear magnetic resonance

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. James Hinton, the director of the NMR Core facility has extensive experience in both solution and solid-state NMR spectroscopy of proteins and peptides. New investigators T.K.S. Kumar and Paul Adams are also highly trained in protein NMR spectroscopy, and have established numerous consultations and collaborations with other COBRE investigators to solve their protein structures. The NMR core facility includes new 700 MHz and 500 MHz NMRs with cryoprobes, as well as a 500 MHz NMR, and two 300 MHz solid-state NMRs. The 700 MHz NMR with cryoprobe was the first to be installed in the US, and offers significant advantages in sensitivity for the study of large proteins. COBRE investigators have been active in writing grant proposals to fund these NMR spectrometers. Of the total cost of $2,884,196, the COBRE grant provided $1,590,000, while other grants and institutional funds provided $1,294196. The University has committed institutional funds for 100% salaries of electrical engineer Marvin Leister and electrician K.Z. Shein to maintain and operate the NMR core facility. The University has also committed matching funds to purchase a 1.7 mm microcryoprobe for the 700 MHz NMR that offers a 14-fold increase in sensitivity for protein NMR.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 詹姆斯欣顿，核磁共振核心设施的主任在蛋白质和肽的溶液和固态核磁共振光谱学方面拥有丰富的经验。 新的调查员T.K.S. Kumar和Paul亚当斯在蛋白质NMR光谱学方面也受过严格的训练，并与其他COBRE研究人员建立了许多磋商和合作，以解决他们的蛋白质结构。 NMR核心设施包括新的700 MHz和500 MHz NMR（带冷冻探针），以及一台500 MHz NMR和两台300 MHz固态NMR。 带有冷冻探针的700 MHz NMR是第一个在美国安装的，并且在大蛋白质研究的灵敏度方面具有显着优势。 COBRE的研究人员一直在积极撰写资助这些NMR光谱仪的拨款提案。 在2，884，196美元的总费用中，COBRE赠款提供了1，590，000美元，其他赠款和机构资金提供了1，294，196美元。 该大学已承诺为电气工程师Marvin Leister和电工K.Z.提供100%的工资。Shein负责维护和操作NMR核心设施。 该大学还承诺提供配套资金，为700 MHz NMR购买1.7 mm微型冷冻探针，使蛋白质NMR的灵敏度提高14倍。