Dynamics of Hair Bundle Proteins

毛细胞机械传导是我们听觉和平衡感的基础过程。的焦点 Shin实验室研究的是介导极其敏感的机械传导过程的蛋白质。 应激因素如反应性自由基和机械过度刺激会损害毛细胞蛋白， 这就提出了这些蛋白质是如何修复或替换的问题。我们的假设是 保持毛细胞蛋白质的完整性是蛋白质的恒定周转。因此，我对 毛细胞蛋白质的动态周转，特别是在响应有害影响，如氧化应激和 噪音损害我目前的ROO建议的目标是使用质谱分析代谢 标记的蛋白质和毛束蛋白的体内成像以研究毛细胞的更新和动力学 proteins. 听力器官的健康取决于其分子的不断更新， 件.这项拟议中的研究使用质谱和成像技术来解决 有害的环境刺激，如噪音损伤，是否会影响蛋白质的周转 对听力很重要。本研究结果将有助于开发预防和恢复听力的方法 人类的损失。

Hair cell mechanotransduction is the process that underlies our senses of hearing and balance. The focus of the Shin lab is the study of proteins that mediate the exquisitely sensitive mechanotransduction process. Stress factors such as reactive radicals and mechanical overstimulation can damage hair cell proteins, raising the question how these proteins are repaired or replaced. Our hypothesis is that the main strategy for maintaining integrity of hair ceil proteins is a constant turnover of proteins. I am therefore interested in the dynamic turnover of hair ceil proteins, especially in response to harmful impacts such as oxidative stress and noise damage. The goal of my present ROO proposal is to use mass spectrometry analysis of metabolically labeled proteins and in vivo imaging of hair bundle proteins to study turnover and dynamics of hair cell proteins. Lay summary: The health of the hearing organ depends on the constant replacement of its molecular components. The proposed study uses mass spectrometry and imaging technologies to address the question whether harmful environmental stimuli such as noise damage can influence the turnover of proteins important for hearing. The results of this study wiii help to develop methods to prevent and restore hearing losses in humans.