ShEEP Request for Leica SP8 Confocal Microscope with Light Sheet

ShEEP 请求购买带光片的 Leica SP8 共焦显微镜

• 批准号: 9795347
• 负责人: ALDEBARAN M HOFER
• 金额: --
• 依托单位: VA BOSTON HEALTH CARE SYSTEM
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9795347
• 关键词: Alzheimer' s Disease; Animals; Area; Biosensor; Boston; Brain; Brain region; Cells; Cellular Structures; Cilia; Collaborations; Collection; Data; Detection; Disease; Equipment; Event; Fees; Fostering; Funding; Healthcare Systems; Human; Hybrids; Image; Image Enhancement; Individual; Institution; Intelligence; Lasers; Light; Lightning; Maintenance; Measurement; Microscope; Microscopy; Modeling; Monitor; Neurodegenerative Disorders; Neurology; Noise; Organelles; Output; Pathology; Reporter; Research; Research Personnel; Resolution; Role; Sampling; Scanning; Schedule; Sheep; Slice; Speed; Stem cells; Structure; Surveys; System; Techniques; Technology; Thick; Three-Dimensional Image; Tissues; Training; data acquisition; design; detector; flexibility; fluorophore; high resolution imaging; improved; instrument; instrumentation; live cell imaging; millisecond; neural circuit; new technology; optogenetics; photomultiplier; protein biomarkers; quantum; relating to nervous system; response; solid state; yeast two hybrid system

This proposal describes a request for funds to purchase the Leica SPS confocal system with White Light laser and Lightning technology, and Light Sheet capabilities. The proposed equipment is a state-of-the-art true confocal system designed to provide high-quality-microscopy of small and large samples with increased sensitivity and faster throughput. The main group of users have active collaborations and have aligned areas of study, mostly concentrating in the field of neurology and neurodegenerative diseases. The addition of the Light Sheet microscopy technology will allow VA-funded investigators at the VA Boston Healthcare System (VABHS) to rapidly acquire high-resolution 3-D images of large sections of human and animal brain samples. The system will also allow researchers to perform studies that require super-resolution imaging. The present application describes our need for the proposed equipment, its technical specifications, information on how it can improve VA-funded research, and a plan for its administration.

该提案描述了为购买具有白光激光和闪电技术的徕卡SPS共聚焦系统以及光片功能的资金请求。该设备是一种最先进的真正共聚焦系统，旨在提供高质量的小型和大型样品显微镜，具有更高的灵敏度和更快的吞吐量。主要的用户群体有积极的合作和一致的研究领域，主要集中在神经病学和神经退行性疾病领域。光片显微镜技术的增加将使VA资助的VA波士顿医疗保健系统（VABHS）的研究人员能够快速获取人类和动物大脑样本的大块高分辨率3d图像。该系统还将允许研究人员进行需要超分辨率成像的研究。目前的申请描述了我们对拟议设备的需求，它的技术规格，关于如何改善va资助的研究的信息，以及它的管理计划。