ShEEP Request for Leica BOND RX

我们正在申请购买徕卡债券RX工具的资金。该仪器使研究人员能够 使用定制协议通过各种测试类型自动分析基因或蛋白质表达，包括 免疫荧光、免疫组织化学、荧光/显色原位杂交、酪胺信号 扩增、microRNA检测、分支DNA分析、末端脱氧核苷酸转移酶dUTP缺口末端 标记试验、循环肿瘤细胞鉴定、多重培养和其他新兴试验。无论是俄克拉荷马州 城市退伍军人医疗保健系统和俄克拉荷马大学附属健康科学中心都有这样的 能够对细胞或组织中的基因或蛋白质进行高精度、自动化定位分析的仪器。 因此，对于俄克拉何马城弗吉尼亚州的科学界来说，这是一个真正独特和有价值的资源 医疗保健系统。徕卡债券RX将由9名调查人员使用，其中6人获得奖励，3人提交 优异奖助金、1个职业发展奖和1个待提交的功勋奖助金。这台设备将会 对所有对退伍军人健康重要的研究领域产生广泛影响，包括：探索 应激后脑回路敏感化相关基因表达的潜在变化 慢性疼痛；确定与坏死性下垂和衰老相关的基因表达的细胞特异性变化； 评估导致肌肉萎缩的脊髓中基因和蛋白表达的变化；特征 导致癌细胞对铂类药物产生耐药性的基因和蛋白质； DCLK1+细胞基因和蛋白表达变化及其在肝细胞癌中的作用分析 测量可能与慢性疼痛有关的大脑回路中基因表达的变化；确定 衰老过程中海马细胞表观基因组变化的功能效应；基因表达特征 个别分泌免疫球蛋白的B细胞渗入唾液腺；确定运动对基因的影响 骨关节炎模型中背根神经节和滑膜组织的表达。

We are requesting funds the purchase a Leica BOND RX instrument. The instrument allows researchers to use custom protocols for automated analysis of gene or protein expression via a variety of test types including immunofluorescence, immunohistochemistry, fluorescence/chromogenic in situ hybridization, tyramide signal amplification, microRNA detection, branched DNA assays, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, circulating tumor cell identification, multiplexing, and other emerging tests. Neither the Oklahoma City VA Health Care System nor the affiliated University of Oklahoma Health Sciences Center have such an instrument that enables high precision, automated analysis of gene or protein localization from cells or tissues. Therefore, this is a truly unique and valuable resource for the scientific community at the Oklahoma City VA Health Care System. The Leica BOND RX will be used by nine investigators with 6 Merit grants, 3 submitted Merit grants, 1 Career Development Award, and 1 pending Merit grant submission. This piece of equipment will have a broad impact on all areas of research important to the health of Veterans, including: exploring the underlying changes in gene expression involved in sensitization of brain circuitry following stress associated with chronic pain; identifying cell-specific changes in gene expression associated with necroptosis and aging; evaluating gene and protein expression changes in the spinal cord resulting in muscle atrophy; characterizing the genes and proteins responsible for the development of resistance to platinum-based drugs in cancer cells; analysis of changes in gene and protein expression in DCLK1+ cells and their role in hepatocellular cancer; measuring changes in gene expression within brain circuits potentially involved in chronic pain; determining the functional effects of epigenomic changes in hippocampal cells during aging; characterizing gene expression of individual IG-secreting B cells as they infiltrate the salivary gland; determining the effect of exercise on gene expression in the dorsal root ganglia and synovial tissue in models of osteoarthritis.