ShEEP Request for Leica BOND RX

SheEEP 请求 Leica BOND RX

基本信息

项目摘要

We are requesting funds the purchase a Leica BOND RX instrument. The instrument allows researchers to use custom protocols for automated analysis of gene or protein expression via a variety of test types including immunofluorescence, immunohistochemistry, fluorescence/chromogenic in situ hybridization, tyramide signal amplification, microRNA detection, branched DNA assays, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, circulating tumor cell identification, multiplexing, and other emerging tests. Neither the Oklahoma City VA Health Care System nor the affiliated University of Oklahoma Health Sciences Center have such an instrument that enables high precision, automated analysis of gene or protein localization from cells or tissues. Therefore, this is a truly unique and valuable resource for the scientific community at the Oklahoma City VA Health Care System. The Leica BOND RX will be used by nine investigators with 6 Merit grants, 3 submitted Merit grants, 1 Career Development Award, and 1 pending Merit grant submission. This piece of equipment will have a broad impact on all areas of research important to the health of Veterans, including: exploring the underlying changes in gene expression involved in sensitization of brain circuitry following stress associated with chronic pain; identifying cell-specific changes in gene expression associated with necroptosis and aging; evaluating gene and protein expression changes in the spinal cord resulting in muscle atrophy; characterizing the genes and proteins responsible for the development of resistance to platinum-based drugs in cancer cells; analysis of changes in gene and protein expression in DCLK1+ cells and their role in hepatocellular cancer; measuring changes in gene expression within brain circuits potentially involved in chronic pain; determining the functional effects of epigenomic changes in hippocampal cells during aging; characterizing gene expression of individual IG-secreting B cells as they infiltrate the salivary gland; determining the effect of exercise on gene expression in the dorsal root ganglia and synovial tissue in models of osteoarthritis.
我们正在申请购买Leica BOND RX仪器的资金。该仪器允许研究人员 使用定制方案通过各种测试类型自动分析基因或蛋白质表达, 免疫荧光,免疫组织化学,荧光/显色原位杂交,酪胺信号 扩增,microRNA检测,分支DNA测定,末端脱氧核苷酸转移酶dUTP缺口末端 标记分析、循环肿瘤细胞鉴定、多重检测和其他新兴检测。俄克拉荷马州 城市VA医疗保健系统也没有附属的俄克拉荷马州健康科学中心大学有这样一个 一种能够对细胞或组织中的基因或蛋白质进行高精度、自动化分析的仪器。 因此,这是一个真正独特的和宝贵的资源,为科学界在俄克拉荷马州城市弗吉尼亚州 卫生保健系统。Leica BOND RX将由9名研究者使用,其中6名获得优异赠款,3名提交 优异赠款,1个职业发展奖,1个待提交的优异奖。这台设备将 对退伍军人健康重要的所有研究领域产生广泛影响,包括:探索 与应激相关的脑回路敏化相关的基因表达的潜在变化 慢性疼痛;鉴定与坏死性凋亡和衰老相关的基因表达的细胞特异性变化; 评估导致肌肉萎缩的脊髓中的基因和蛋白质表达变化;表征 负责癌细胞对铂类药物产生耐药性的基因和蛋白质; 分析DCLK 1+细胞中基因和蛋白表达的变化及其在肝细胞癌中的作用; 测量可能与慢性疼痛有关的脑回路内基因表达的变化; 衰老过程中海马细胞表观基因组变化的功能影响;表征海马细胞表观基因组的基因表达, 单个的分泌免疫球蛋白的B细胞浸润唾液腺;确定运动对基因的影响 在骨关节炎模型的背根神经节和滑膜组织中表达。

项目成果

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Beverley Greenwood-Van Meerveld其他文献

Beverley Greenwood-Van Meerveld的其他文献

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{{ truncateString('Beverley Greenwood-Van Meerveld', 18)}}的其他基金

BLR&D Research Career Scientist Award Application
BLR
  • 批准号:
    10046730
  • 财政年份:
    2016
  • 资助金额:
    --
  • 项目类别:
BLR&D Research Career Scientist Award Application
BLR
  • 批准号:
    9231123
  • 财政年份:
    2016
  • 资助金额:
    --
  • 项目类别:
Central Mechanisms Modulating Visceral Sensitivity
调节内脏敏感性的中枢机制
  • 批准号:
    8543970
  • 财政年份:
    2013
  • 资助金额:
    --
  • 项目类别:
Understanding Pain of Gastrointestinal Origin in Women that Serve in OEF/OIF
了解 OEF/OIF 女性胃肠道疼痛
  • 批准号:
    9033203
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
Understanding Pain of Gastrointestinal Origin in Women that Serve in OEF/OIF
了解 OEF/OIF 女性胃肠道疼痛
  • 批准号:
    8254312
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
Understanding Pain of Gastrointestinal Origin in Women that Serve in OEF/OIF
了解 OEF/OIF 女性胃肠道疼痛
  • 批准号:
    9210532
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
Understanding Pain of Gastrointestinal Origin in Women that Serve in OEF/OIF
了解 OEF/OIF 女性胃肠道疼痛
  • 批准号:
    8398974
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
Understanding Pain of Gastrointestinal Origin in Women that Serve in OEF/OIF
了解 OEF/OIF 女性胃肠道疼痛
  • 批准号:
    8696838
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
Understanding Pain of Gastrointestinal Origin in Women that Serve in OEF/OIF
了解 OEF/OIF 女性胃肠道疼痛
  • 批准号:
    8142489
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:

相似海外基金

The Aging Kidney Anatomy Study
衰老肾脏解剖学研究
  • 批准号:
    9243240
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
The macro- and micro- anatomy and pathology of the aging kidney
衰老肾脏的宏观和微观解剖学及病理学
  • 批准号:
    8022523
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
The macro- and micro- anatomy and pathology of the aging kidney
衰老肾脏的宏观和微观解剖学及病理学
  • 批准号:
    8425058
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
The macro- and micro- anatomy and pathology of the aging kidney
衰老肾脏的宏观和微观解剖学及病理学
  • 批准号:
    8602520
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
The macro- and micro- anatomy and pathology of the aging kidney
衰老肾脏的宏观和微观解剖学及病理学
  • 批准号:
    8223232
  • 财政年份:
    2011
  • 资助金额:
    --
  • 项目类别:
AUDITORY ANATOMY IN AGING RATS WITH EXTENDED LIFESPANS
寿命延长的老龄大鼠的听觉解剖学
  • 批准号:
    3117129
  • 财政年份:
    1988
  • 资助金额:
    --
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GROWTH AND AGING--ANATOMY OF SUBSTANTIA NIGRA
生长与衰老--黑质的解剖
  • 批准号:
    3476748
  • 财政年份:
    1987
  • 资助金额:
    --
  • 项目类别:
GROWTH AND AGING--ANATOMY OF SUBSTANTIA NIGRA
生长与衰老--黑质的解剖
  • 批准号:
    3476751
  • 财政年份:
    1987
  • 资助金额:
    --
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GROWTH AND AGING--ANATOMY OF SUBSTANTIA NIGRA
生长与衰老--黑质的解剖
  • 批准号:
    3476752
  • 财政年份:
    1987
  • 资助金额:
    --
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GROWTH AND AGING--ANATOMY OF SUBSTANTIA NIGRA
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  • 批准号:
    3476753
  • 财政年份:
    1987
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    --
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