The Supramolecular Architecture of the Postsynaptic Density

这个项目致力于人类大脑皮层神经元的结构。Aim 1使用来自 切除颞叶，进行高分辨率定量电子显微镜研究， 兴奋性突触的结构，包括识别类别的中间神经元上的突触。使用相同的 生物材料，目标2进行定量免疫金电子显微镜，以确定架构的生物材料， 突触后密度中丰富的许多不同分子，集中在突变的蛋白质上， 涉及神经精神和神经发育疾病，尤其是精神分裂症。目标3执行 其工作类似于针对目标1和2所规定的，但集中于体外培养的皮质类器官。这些 结果将使我们有可能比较培养中突触的结构和分子组织， 完整大脑中的突触

This project addresses the architecture of neuropil in the human cerebral cortex. Aim 1 uses material from surgically-resected temporal lobe to perform high-resolution quantitative electron microscopic studies of the structure of excitatory synapses, including synapses onto identified classes of interneurons. Using the same biological material, Aim 2 performs quantitative immunogold electron microscopy to define the architecture of a number of different molecules abundant in the postsynaptic density, focusing on proteins whose mutations are implicated in neuropsychiatric and neurodevelopmental disease, especially schizophrenia. Aim 3 performs work similar to that specified for Aims 1 and 2, but focusing on cortical organoids cultured in vitro. These results will make it possible to compare the structure and molecular organization of synapses in culture with synapses in intact brain.