Drosophila as a model to dissect the rise and role of calcium in egg activation.

果蝇作为模型来剖析钙在卵子激活中的上升和作用。

基本信息

  • 批准号:
    9323477
  • 负责人:
  • 金额:
    $ 19.15万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-07-29 至 2020-06-30
  • 项目状态:
    已结题

项目摘要

Project Summary A differentiated, mature oocyte is “activated” to become totipotent and to begin embryo development through an increase in its cytoplasmic free Ca2+ levels. In mammals, this increase is initially induced by the fertilizing sperm, which stimulates release of Ca2+ from internal stores. However, recent studies have shown that subsequent Ca2+ oscillations that are essential for mammalian egg activation require influx of external Ca2+ from the environment into the oocyte, through as yet unknown channels and mechanisms. Although Drosophila had not been considered a good model for these processes because its eggs activate prior to fertilization, our recent work has shown that Drosophila egg activation also involves a local Ca2+ influx. This influx is followed by a wave of increased Ca2+ that sweeps through the egg cytoplasm, using mechanisms like those used by mammalian oocytes. We showed that the initial Ca2+ influx in Drosophila occurs at the oocyte poles, through mechanosensitive channels – most likely conserved channels in the TRP family. We propose to establish Drosophila as a new model for the Ca2+-based phase of egg activation. We will use this model to identify the channels that mediate sperm-independent Ca2+ influx and the conditions that cause Ca2+ channels to be open only at the egg poles. We will also determine whether the increased Ca2+ mediates the changes to the oocyte phosphoproteome that normally accompany egg activation, and whether the Ca2+ signal transducer calcineurin is required for these phosphoproteome changes. We expect the results of our studies to be generalizable to mechanisms in mammalian egg activation. Moreover, conserved proteins with Ca2+- dependent phosphomodifications will be candidates for future testing as markers for assessing human ART conditions, oocyte quality and aiding the diagnosis of infertilities that cannot respond to current ART methods.
项目摘要 一个分化的成熟卵母细胞被“激活”成为全能的,并开始开始胚胎 通过增加其细胞质游离Ca 2+水平的发展。在哺乳动物中, 增加最初是由精子引起的,它刺激Ca 2+从 内部存储。然而,最近的研究表明,随后的Ca 2+振荡 哺乳动物卵子激活所必需的钙离子需要从卵细胞中流入, 通过未知的渠道和机制,环境进入卵母细胞。 尽管果蝇并不被认为是这些过程的良好模型 因为果蝇的卵在受精前就被激活了,我们最近的研究表明, 卵的激活还涉及局部Ca 2+内流。这种涌入之后是一波 增加了钙离子在卵细胞质中的流动, 哺乳动物卵母细胞所使用的。我们发现,果蝇中最初的Ca 2+内流发生在 在卵母细胞的两极,通过机械敏感通道-最有可能是保守的 TRP家族中的通道。我们建议建立果蝇作为一个新的模式, 卵激活的钙基阶段。我们将使用这个模型来识别 介导精子非依赖性Ca 2+内流和导致Ca 2+通道 只在蛋杆处开放。我们还将确定增加的Ca 2+是否 介导通常伴随卵子的卵母细胞磷酸化蛋白质组的变化 激活,以及这些是否需要Ca 2+信号转导钙调磷酸酶 磷酸化蛋白质组变化。我们希望我们的研究结果能够推广到 哺乳动物卵子激活的机制。此外,保守的蛋白质与Ca 2 +- 依赖性磷酸化修饰将是未来测试的候选者, 评估人类ART条件、卵母细胞质量和辅助诊断不孕症 无法应对现有的ART方法。

项目成果

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Mariana Federica Wolfner其他文献

Mariana Federica Wolfner的其他文献

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{{ truncateString('Mariana Federica Wolfner', 18)}}的其他基金

Determining the Role of the Conserved TRPM Ion Channel in Egg Activation, Using the Drosophila Model
使用果蝇模型确定保守 TRPM 离子通道在卵激活中的作用
  • 批准号:
    10271276
  • 财政年份:
    2020
  • 资助金额:
    $ 19.15万
  • 项目类别:
Drosophila as a model to dissect the rise and role of calcium in egg activation.
果蝇作为模型来剖析钙在卵子激活中的上升和作用。
  • 批准号:
    9164791
  • 财政年份:
    2016
  • 资助金额:
    $ 19.15万
  • 项目类别:
New conserved candidate for egg activation and early embryogenesis in Drosophila.
果蝇卵激活和早期胚胎发生的新保守候选者。
  • 批准号:
    8531438
  • 财政年份:
    2013
  • 资助金额:
    $ 19.15万
  • 项目类别:
Actions of Seminal Proteins in Mated Drosophila Females
精液蛋白在交配果蝇雌性中的作用
  • 批准号:
    7862948
  • 财政年份:
    2009
  • 资助金额:
    $ 19.15万
  • 项目类别:
ACTIONS OF SEMINAL PROTEINS IN MATED DROSOPHILA FEMALES
精液蛋白在交配雌性果蝇中的作用
  • 批准号:
    6651927
  • 财政年份:
    2000
  • 资助金额:
    $ 19.15万
  • 项目类别:
Actions of Seminal Proteins in Mated Drosophila Females
精液蛋白在交配果蝇雌性中的作用
  • 批准号:
    7346974
  • 财政年份:
    2000
  • 资助金额:
    $ 19.15万
  • 项目类别:
Actions of Seminal Proteins in Mated Drosophila Females
精液蛋白在交配果蝇雌性中的作用
  • 批准号:
    7002329
  • 财政年份:
    2000
  • 资助金额:
    $ 19.15万
  • 项目类别:
Actions of Seminal Proteins in Mated Drosophila Females
精液蛋白在交配果蝇雌性中的作用
  • 批准号:
    10266142
  • 财政年份:
    2000
  • 资助金额:
    $ 19.15万
  • 项目类别:
Actions of Seminal Proteins in Mated Drosophila Females
精液蛋白在交配果蝇雌性中的作用
  • 批准号:
    10443880
  • 财政年份:
    2000
  • 资助金额:
    $ 19.15万
  • 项目类别:
Actions of Seminal Proteins in Mated Drosophila Females
精液蛋白在交配果蝇雌性中的作用
  • 批准号:
    6917535
  • 财政年份:
    2000
  • 资助金额:
    $ 19.15万
  • 项目类别:

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