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Cell-Type Specific Regulation of Dendritic mRNA in the Hippocampus

海马树突 mRNA 的细胞类型特异性调控

基本信息

批准号：
9814980
负责人：
Shannon Farris
金额：
$ 24.9万
依托单位：
VIRGINIA POLYTECHNIC INST AND ST UNIV
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-11-01 至 2021-10-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9814980
关键词：
Affect Alternative Splicing Award Behavior Bioinformatics Biology Brain Cell physiology Cells Clinical Trials Code Communication Complement Complex Cues Dendrites Development Drug Targeting Educational workshop Exhibits Extramural Activities FMR1 Faculty Funding Future Gene Expression Genetic Genetic Transcription Goals Grant Hippocampus (Brain)Individual Institution Investigation K-Series Research Career Programs Knockout Mice Knowledge Laboratories Learning Long-Term Potentiation Mediating Memory Mental Health Mentors Mentorship Messenger RNA Methods Molecular Mus Neurodevelopmental Disorder Neurologic Symptoms Neuromodulator Neurons Oxytocin Pathway interactions Pattern Peptides Pharmaceutical Preparations Phase Play Positioning Attribute Process Protein Biosynthesis Proteins RNA RNA Stability RNA-Binding Proteins Regulation Research Research Training Resources Role Schizophrenia Social Behavior Social support Synapses Synaptic plasticity Techniques Training Trans-Activators Transcript Translating Translational Regulation Translations Universities Untranslated RNA Variant Vasopressins Work autism spectrum disorder base behavioral plasticity career cell type cost design effective therapy genome-wide hands on instruction hippocampal subregions innovation insight laser capture microdissection mRNA Stability meetings mouse model neuropsychiatric disorder new therapeutic target next generation sequencing novel novel therapeutics programs response ribosome profiling skills social social deficits social learning summer student synaptic function tenure track therapeutic candidate therapeutic target tool transcriptome translatome

项目摘要

PROJECT SUMMARY The overall goal of this career development award is to acquire the essential research and professional skills needed to establish an extramurally funded research group to investigate the post-transcription mechanisms underlying social memory. Specifically, the grant builds upon previous training in the field of mRNA regulation in neuronal dendrites and adds training in essential and innovative techniques, such as bioinformatics analyses and ribosome profiling, to further pursue genome-wide translational regulation in neuronal cell-types important for social memory. The grant brings together a number of local experts to synergistically support both the career and research training. Formal training in next generation sequencing analyses will be acquired through didactic coursework and hands-on instruction at the primary institution. Training in translational profiling methods and analyses will be taught under the guidance of a co-mentor at a local top university. Laboratory management, grantsmanship and scientific communication skills will be attained through workshops, short courses and presentations at national and regional scientific meetings, respectively. Lastly, mentorship skills will be honed through a summer student research program at the primary institution. Upon successful completion of the training portion of the grant, it is expected that the awardee will be highly competitive for a tenure-track faculty position at research-intensive university. During the independent phase of the award, the research plan expands to include a genetic targeting approach to interrogate how mRNA stability in specific cell-types contributes to synaptic and behavioral plasticity underlying social learning. Work from this grant will expand our knowledge of the post-transcriptional mechanisms regulating translation in neurons and potentially identify novel therapeutic candidates for the treatment of neuropsychiatric disorders with abnormal sociocognitive processing, such as autism spectrum disorders and schizophrenia.

项目摘要这个职业发展奖的总体目标是获得必要的研究和专业技能需要建立一个由外部资助的研究小组来研究转录后机制潜在的社会记忆。具体来说，该基金建立在mRNA调控领域以前的培训基础上在神经元树突，并增加了基本和创新技术的培训，如生物信息学分析，和核糖体分析，以进一步追求神经元细胞类型的全基因组翻译调控，社会记忆。该赠款汇集了一些当地专家，以协同支持职业和研究培训。下一代测序分析的正式培训将通过在初级教育机构开展教学课程和实践教学。翻译谱分析培训方法和分析将在当地顶尖大学的共同导师的指导下教授。实验室管理、培训和科学交流技能将通过讲习班、短期培训和短期培训获得。分别在国家和区域科学会议上举办培训班和作专题介绍。最后，指导技能将通过在主要机构的暑期学生研究计划进行磨练。一旦成功在完成赠款的培训部分后，预计获奖者将具有很强的竞争力，研究型大学终身教职。在该奖项的独立阶段，研究计划扩大到包括遗传靶向方法研究特定细胞类型中mRNA的稳定性如何影响突触和行为可塑性潜在的社会学习。这项资助的工作将扩大我们对转录后基因的了解，调节神经元翻译的机制，并可能为神经元翻译识别新的治疗候选物。治疗伴有异常社会认知处理的神经精神疾病，例如自闭症谱系精神障碍和精神分裂症。