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Understanding and using microbial conductive nanowires

了解和使用微生物导电纳米线

基本信息

批准号：
10215137
负责人：
Fengbin Wang
金额：
$ 9.49万
依托单位：
UNIVERSITY OF VIRGINIA
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-01 至 2023-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10215137
关键词：
Address Algorithms Amaze Anaerobic Bacteria Antibodies Archaea Bacteria Biochemical Biocompatible Materials Bioinformatics Biophysical Process Biosensor Cells Collaborations Cryoelectron Microscopy Cytochrome c Group Cytochromes Data Electron Microscopy Electron Transport Electrons Fiber Filament Fimbriae Proteins Future Genetic Geobacter Growth Heme Hydrogen Knowledge Label Lead Measurement Measures Medical Mentors Messenger RNA Microbe Microscopic Modeling Molecular Morphology Organism Oxidants Oxidation-Reduction Peptides Pilum Point Mutation Polymers Prokaryotic Cells Proteins Proteomics Pyrobaculum Recombinants Resolution Respiration Role Scaffolding Protein Source Structure Surface Techniques Validation Work appendage base biophysical properties biophysical techniques cryogenics design extracellular heme C imaging approach insight interest microbial monomer mutant nanowire novel scaffold synthetic biology

项目摘要

Abstract Long-range (>10 μm) transport of electrons along networks of G. sulfurreducens protein filaments, known as microbial nanowires, has been invoked to explain a wide range of globally important redox phenomena. The remarkable electronic conduction capability of those nanowires has sparked a great deal of interest in the medical application space, such as for building biocompatible materials and biosensor. For more than a decade, G. sulfurreducens nanowires were thought to be bacterial type IV pili, supported by many indirect genetic and biochemical observations. Recently we showed that these conductive nanowires are not made of type IV pilins. Instead, these structures are a polymerized multi-heme c-type cytochrome, OmcS, which have never been characterized before. The OmcS filament model is consistent with the known roles of OmcS in Geobacter respiration, but our knowledge of cytochrome appendages is still very limited. This study aims at addressing fundamental scientific questions about cytochrome filaments in respiring prokaryotes as well as applying our discoveries into the general medical field. Specifically, I will: A) identify and characterize novel cytochrome filaments in bacterial and archaeal strains, through bioinformatics algorithms followed by microscopic validation. B) Then I will study the conduction mechanism of these filaments by high resolution cryogenic electron microscopy (cryo-EM) and conductivity measurement. C) Finally, based on these new insights into cytochrome filaments, I will create a novel design for a self-assembled conductive nanowire. These nanowires may be derived directly from a novel cytochrome filament or may contain a peptide/protein based self-assembled scaffold core with soluble cytochromes appended to the outer surface. The results will advance our understanding of cytochrome nanowires, as well as generating self-assembling nanowire scaffolds that may be used in many future biomedical applications.

摘要