Immunopathology Core

项目摘要 免疫病理学核心计划（IPC）的具体目标是提供病理学 和免疫学支持COBRE和其他俄克拉荷马州中心的呼吸和 传染病（OCRID）研究人员。IPC提供的服务包括广泛的 一系列病理学支持，包括大体尸检和组织收集、组织 常规和特殊冷冻或石蜡包埋组织的处理、切片 染色，免疫组织化学（IHC），免疫荧光（IFC），和协调 辅助服务（临床病理学、细菌学、病毒学、毒理学）。免疫 在疾病发展中重要的反应的特征在于评估细胞， 介导的免疫（ELISPOT，细胞内IFNγ的流式细胞术）和表型 病变内炎性细胞的鉴定（IFC、IHC、流式细胞术）。的IPC 还将与动物模型核心（AMC）和分子生物学核心协调 (MBC)协助研究者进行体液免疫（血清学、ELISA）和定量 细胞因子基因表达（real-time PCR）。为了实现这一目标，IPC 由三名具有动物模型经验的委员会认证病理学家组成， 呼吸道/传染病研究和一个经验丰富，广泛的交叉训练 技师IPC拥有作为基础的资源， 体制承诺，确保IPC发展成为自我维持的设施 是OCRID研究基础设施不可或缺的一部分。

PROJECT SUMMARY The specific aim of the Immunopathology Core Program (IPC) is to provide pathology and immunology support to COBRE and other Oklahoma Center for Respiratory and Infectious Diseases (OCRID) investigators. Services provided by the IPC include a broad range of pathology support including gross necropsy and tissue collection, tissue processing, sectioning of either frozen or paraffin-embedded tissues, routine and special stains, immunohistochemistry (IHC), immunofluorescence (IFC), and coordination of ancillary services (clinical pathology, bacteriology, virology, toxicology). Immune responses important in the development of disease are characterized by evaluation of cell- mediated immunity (ELISPOT, flow cytometry for intracellular IFNγ) and phenotypic identification of inflammatory cells within lesions (IFC, IHC, flow cytometry). The IPC will also coordinate with the Animal Models Core (AMC) and Molecular Biology Core (MBC) to assist investigators with humoral immunity (serology, ELISA) and quantitation of cytokine gene expression (real-time PCR) respectively. To achieve this aim, the IPC is staffed by three board-certified pathologists with experience in animal models and respiratory/infectious disease research and an experienced, extensively cross-trained technician. The IPC has the resources in place to serve as the foundation and a strong institutional commitment to ensure development of the IPC into a self-sustaining facility integral to the research infrastructure of OCRID.