Gene identification and functional characterization for metabolism-based herbicide resistance in Lolium rigidum

黑麦草基于代谢的除草剂抗性的基因鉴定和功能表征

• 批准号: LP140100909
• 负责人: Em/Prof Stephen Powles
• 金额: $ 23.09万
• 依托单位: The University of Western Australia
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-05-08 至 2018-05-07
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP140100909
• 关键词: Gene; identification; functional; characterization; metabolism

Evolution of multiple herbicide resistance is widespread in Lolium rigidum in Australia. This resistance is very often endowed by enhanced rates of herbicide metabolism (metabolic resistance) involving cytochrome P450. This project aims to identify, clone and characterise important herbicide-metabolising P450 and other genes from multiple herbicide-resistant L. rigidum biotypes, and develop transcriptional and biochemical markers for metabolic resistance diagnosis. Herbicide-metabolising gene discovery, characterisation and marker development will greatly extend the currently limited knowledge and understanding of metabolic resistance and help achieve sustainable weed management.

在澳大利亚硬直黑麦草中，除草剂多重抗性的进化是普遍的。这种抗性通常是由涉及细胞色素P450的除草剂代谢（代谢抗性）的增强速率赋予的。本项目旨在从多种抗除草剂的L. rigidum生物型，并开发用于代谢抗性诊断的转录和生化标记。除草剂代谢基因的发现、表征和标记物的开发将极大地扩展目前有限的知识和对代谢抗性的理解，并有助于实现可持续的杂草管理。