Quantitative Proteomics to Develop Robust Senescence-Related Biomarkers for Aging

定量蛋白质组学开发强大的衰老相关生物标志物

• 批准号: 10403934
• 负责人: Birgit Schilling
• 金额: $ 74.16万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-30 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10403934
• 关键词: Adopted; Affect; Age; Age-Years; Aging; American; Animals; Atherosclerosis; Baltimore; Biological; Biological Aging; Biological Assay; Biological Markers; Biology of Aging; Cardiovascular Diseases; Cell Aging; Cell Culture Techniques; Cells; Cessation of life; Chronic; Clinical; Complex; Degenerative polyarthritis; Disease; Health; Health Status; Healthcare Systems; Heart; Human; Impaired cognition; Inflammation; Intervention; Kidney Failure; Laboratories; Link; Longitudinal Studies; Malignant Neoplasms; Mass Spectrum Analysis; Measures; Modernization; Molecular; Monitor; Mus; Neurodegenerative Disorders; Normal tissue morphology; Older Population; Organ; Organism; Osteoporosis; Outcome; Pathologic; Pathology; Pathway interactions; Patients; Pharmacologic Substance; Phenotype; Plasma; Proteins; Proteomics; Reproducibility; Research; Risk Factors; Sampling; Societies; Stress; Structure; Technology; Tissues; Transgenic Mice; Work; age related; aged; aging demographic; base; biobank; biomarker panel; candidate marker; candidate validation; cardiovascular health; cell injury; cognitive capacity; cohort; driving force; exhaustion; exosome; falls; frailty; functional decline; functional status; high throughput screening; in vivo; insight; mouse model; multiplex assay; novel; novel marker; pre-clinical; predictive marker; predictive tools; response; response biomarker; sarcopenia; senescence; stem cells; tool; tumorigenesis

SUMMARY Aging entails a plethora of changes at multiple levels – affecting molecules, cells, tissues, organs and integrated organisms -- which culminate in a concerted decline in overall health and ultimately death. Aging is also considered a significant risk factor for many conditions and diseases, such as cardiovascular diseases (CVD), atherosclerosis, sarcopenia, osteoporosis, renal failure and neurodegenerative diseases. Whereas significant progress has been made in understanding several of the cell intrinsic molecular pathways and cellular responses that drive aging, it is very challenging to assess the progression of aging and aging-related conditions, and the field is clearly lacking robust biomarkers for aging. We propose to use our pioneering work in cellular senescence in combination with state-of-the-art proteomics technologies to develop robust senescence-related biomarkers of aging. These novel aging biomarkers are expected to become critical research tools both for transforming the way how to look at human aging and for predicting health outcomes based on biological age and not chronological age (years of age). In parallel, we will refine these biomarkers also for animal studies to gain greater insights into mechanisms of Biology of Aging.

总结 衰老需要在多个层面上发生过多的变化-影响分子，细胞，组织，器官和组织。 整合的有机体--最终导致整体健康状况的一致下降并最终死亡。衰老是 也被认为是许多疾病的重要危险因素，如心血管疾病 (CVD)动脉粥样硬化、肌肉减少症、骨质疏松症、肾衰竭和神经变性疾病。而 在理解几种细胞内在分子途径方面已经取得了重大进展， 由于细胞反应驱动衰老，因此评估衰老的进展和与衰老相关的 条件下，该领域显然缺乏强大的衰老生物标志物。我们建议利用我们的开创性工作 结合最先进的蛋白质组学技术， 衰老相关的生物标志物。这些新的衰老生物标志物预计将成为关键 研究工具，既可以改变人们看待人类衰老的方式，也可以预测健康结果 基于生物学年龄而不是实足年龄（年龄）。同时，我们将完善这些生物标志物， 也为动物研究，以获得更深入的了解衰老生物学的机制。