Quantitative Proteomics to Develop Robust Senescence-Related Biomarkers for Aging

摘要 衰老需要在多个层面上发生过多的变化--影响分子、细胞、组织、器官和 整合的有机体--最终导致整体健康水平下降，最终导致死亡。老龄化是 也被认为是许多疾病和疾病的重要危险因素，如心血管疾病 心血管疾病、动脉粥样硬化、骨质疏松症、肾功能衰竭和神经退行性疾病。鉴于 在理解几个细胞内在的分子通路和 推动衰老的细胞反应，评估衰老和衰老相关的进展是非常具有挑战性的 条件，该领域显然缺乏强大的生物标记物的老化。我们打算用我们的开创性工作 与最先进的蛋白质组学技术相结合，开发出强大的 衰老相关的衰老生物标志物。这些新的老化生物标志物有望成为关键 用于改变看待人类老龄化的方式和预测健康结果的研究工具 基于生物年龄，而不是按时间顺序计算的年龄(年龄)。同时，我们将提炼这些生物标志物 也用于动物研究，以更深入地了解衰老的生物学机制。

SUMMARY Aging entails a plethora of changes at multiple levels – affecting molecules, cells, tissues, organs and integrated organisms -- which culminate in a concerted decline in overall health and ultimately death. Aging is also considered a significant risk factor for many conditions and diseases, such as cardiovascular diseases (CVD), atherosclerosis, sarcopenia, osteoporosis, renal failure and neurodegenerative diseases. Whereas significant progress has been made in understanding several of the cell intrinsic molecular pathways and cellular responses that drive aging, it is very challenging to assess the progression of aging and aging-related conditions, and the field is clearly lacking robust biomarkers for aging. We propose to use our pioneering work in cellular senescence in combination with state-of-the-art proteomics technologies to develop robust senescence-related biomarkers of aging. These novel aging biomarkers are expected to become critical research tools both for transforming the way how to look at human aging and for predicting health outcomes based on biological age and not chronological age (years of age). In parallel, we will refine these biomarkers also for animal studies to gain greater insights into mechanisms of Biology of Aging.