"Core D" Clinical Sample and Tumorigenesis Core

核心D摘要 该计划项目的统一目标是定义非编码RNA的生物学功能 伽马疱疹病毒，艾滋病，恶性肿瘤。尽管体外实验仍然是科学研究的核心组成部分 使用临床样本和活体系统的进步和方法对于审问是绝对必要的 正常的生物过程，定义病理的关键决定因素，并开发合理的策略来 与疾病作斗争。核心D(临床样本和肿瘤发生核心)的目的是提供必要的 为项目1、项目2和项目3提供服务，包括临床样本采集和储存(目标1)；组织学 处理、临床病理评分、RNAScope原位杂交和GeoMx数字空间分析 临床肿瘤样本(目标2)；以及使用人源化小鼠、异种移植小鼠和 小鼠病毒肿瘤发生系统(目标3)。因此，核心D将通过利用 确定病毒和宿主生物学相关性的现代体内技术和肿瘤发生模型 项目1、2和3中确定的非编码RNA。

CORE D SUMMARY The unifying goal of this program project is to define the biological function of noncoding RNAs in gammaherpesvirus AIDS malignancies. Though in vitro experimentation remains a core component of scientific advancements, approaches using clinical samples and in vivo systems are absolutely essential for interrogating normal biological processes, defining critical determinants of pathology, and developing rational strategies to combat disease. The purpose of Core D (Clinical Sample and Tumorigenesis core) is to provide essential services for Projects 1, 2 and 3 that include clinical sample acquisition and storage (Aim 1); histological processing, clinical pathology scoring, RNAscope in situ hybridization, and GeoMx digital spatial profiling of clinical tumor samples (Aim 2); and in vivo experimentation using humanized mouse, xenograft mouse, and murine virus tumorigenesis systems (Aim 3). Thus, Core D will aid in the overall goal of the program by utilizing modern in vivo techniques and tumorigenesis models to define the biological relevance of virus and host noncoding RNAs identified in Projects 1, 2 and 3.