High-speed hyperspectral imaging for highly multiplexed immunofluorescence imaging

用于高度多重免疫荧光成像的高速高光谱成像

基本信息

批准号：
10699518
负责人：
GREGORY W FARIS
金额：
$ 30.07万
依托单位：
NUMENTUS TECHNOLOGIES INC.
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10699518
关键词：
Alzheimer&apos s Disease Antibodies Biological Markers Brain Brain imaging Cell physiology Clinical Clinical Medicine Clinical Pathology Collection Complement Complex Consumption Coupled Data Collection Detection Development Diagnosis Disease Experimental Pathology Goals Hour Image Image Analysis Imaging Device Immunofluorescence Immunologic Immunohistochemistry Label Laboratories Laboratory Research Lighting Literature Mass Spectrum Analysis Methods Microscope Mitochondria Modeling Modernization Molecular Target Multiplexed Ion Beam Imaging Mus Nerve Degeneration Neuroanatomy Neurodegenerative Disorders Nuclear Paraffin Embedding Pathology Pathology Report Phase Proteins Protocols documentation RNA Splicing RNA Transport Reporting Research Resolution Ribonucleoproteins Role Scanning Selection for Treatments Slide Speed System Technology Testing Time Tissues Transgenic Mice Translations Work age related brain tissue cost fluorescence imaging fluorophore imaging modality imaging platform imaging system improved in vivo instrument instrumentation mass spectrometric imaging meter mouse model multiplexed imaging neoplastic neuropathology novel operation optical imaging protein TDP-43 sensor tool

项目摘要

PROJECT SUMMARY Pathology using immunohistochemistry (IHC) remains a critical tool in modern clinical medicine. However, the capabilities of routine IHC methods have not kept pace with advances in biomedicine, which require evaluating networks of, e.g., tens of large molecules (typically protein targets) in a tissue context by multiplexing. To overcome this limit, highly multiplexed IHC imaging systems based on mass spectrometry and repeated Immunofluorescence (IF) imaging have been developed. These systems claim theoretical multiplexing of up to 100 targets. However, they are too slow, expensive, and complex, for routine use, and are currently used in a small number of research laboratories. We are working to radically change the capabilities of IF imaging to allow routine detection of tens of molecular targets rapidly and at moderate cost. Our approach builds on our previous work on high-speed hyperspectral imaging coupled with amplified molecular target labeling methods. We will advance our technology and demonstrate its capabilities with a specific research application involving imaging of brain sections from a mouse model of a neuropathology relevant for Alzheimer’s disease (AD). We will make improvements to achieve simpler and faster operation of our technology. We will then image up to 12 targets and demonstrate the ability to extend our approach to tens of targets on a single tissue slide. We expect our instrumentation and protocols will be sufficiently simple, low cost, and rapid to allow routine use in most pathology and research laboratories. !

项目摘要使用免疫组织化学（IHC）的病理学仍然是现代临床医学的关键工具。然而，常规IHC方法的功能尚未跟上生物医学的进步，这需要通过组织环境中评估数十个大分子（通常是蛋白质靶标）的网络多路复用。为了克服这个极限，高度多重的IHC成像系统基于质谱和已经开发了重复的免疫荧光（IF）成像。这些系统声称理论多达100个目标的多路复用。但是，它们太慢，昂贵且复杂，用于常规使用，并且目前用于少量研究实验室。我们正在努力从IF成像的能力彻底更改以常规检测的能力分子目标迅速，成本中等。我们的方法基于我们先前在高速的工作高光谱成像，加上扩增的分子靶标标记方法。我们将通过特定的研究申请提高技术并展示其功能涉及与阿尔茨海默氏病有关的小鼠模型的脑部成像（广告）。我们将进行改进，以实现我们技术的更简单，更快的操作。然后我们会图像最多12个目标，并证明将我们的方法扩展到单个组织上的数十个目标的能力滑动。我们预计我们的仪器和协议将足够简单，低成本，并且迅速允许在大多数病理学和研究实验室中的常规使用。呢