Advanced Imaging Core

项目总结/摘要 高级成像核心为本计划项目组的每个项目提供人员配备， 专业知识，设备和解释技能，需要评估和测试转移的机制， 肺的次要部位。所提供的服务是该核心所独有的，因为它们是由 一些独立实验室在显微镜和活体成像方面取得的进展， 爱因斯坦的Gruss-Lipper生物光子学中心正如研究战略中详细描述的那样， 服务包括1）定制的多激光多光子显微镜; 2）纵向活体成像， 单细胞分辨率; 3）体内肺和其他次级部位成像; 4）体内细胞命运作图; 5）大- 体积高分辨率活体成像（LVHR-IVI）; 6）用于干性的生物传感器库， 休眠，入侵和缺氧; 7）相关显微镜结合活体成像， 组织病理学（IVITA）;以及8）用于固定组织研究的定量数字病理学服务。总体上 使用先进的多光子显微镜;准备动物成像，包括麻醉和 手术;收集的数据的分析和解释需要通过实践发展的技能。 核心A有专门的专家工作人员，将集中管理所需的设备和知识库 确保本计划的成员优先访问所有服务，并正确使用所有服务 项目组以确保所有实验的严谨性和可重复性的方式。调查人员 本PPG的各个项目将首先与高级职员协商，以确定 实验，并准备一个协议，以实现这些目标。这个核心的工作人员将与一个 项目代表执行实验，核心人员提供实践技能 在动物手术和处理、成像和数据收集的苛刻步骤中， 方法的应用以及获得和解释数据所需的质量控制。未经 服务，我们对这些过程的理解将来自模型系统 和/或仅接近肺转移生理学的终末期测定。相比之下，我们的方法 独特地能够提供关于在单细胞水平上的体内行为机制的信息。

Project Summary/Abstract The Advanced Imaging Core provides to each of the projects of this Program Project Group the staffing, expertise, equipment, and interpretive skills needed to evaluate and test mechanisms of metastasis at the secondary site of the lung. The services provided are unique to this Core as they have been assembled from advances in microscopy and intravital imaging made by a number of independent laboratories within Einstein’s Gruss-Lipper Biophotonics center. As described in detail within the research strategy, these services consist of 1) A custom-built multi-laser multiphoton microscope; 2) Longitudinal intravital imaging at single cell resolution; 3) In vivo lung and other secondary site imaging; 4) In vivo cell fate mapping; 5) Large- Volume High-Resolution Intravital Imaging (LVHR-IVI); 6) A repository of biosensors for stemness, dormancy, invasiveness, and hypoxia; 7) Correlative microscopy combining intravital imaging with histopathology (IVITA); and 8) Quantitative digital pathology services for fixed tissue studies. In general, the use of advanced multiphoton microscopy; the preparation of animals for imaging including anesthesia and surgery; and the analysis and interpretation of the data collected require skill developed through practice. With dedicated expert staff members, Core A will centralize the equipment and knowledgebase needed to ensure prioritized access, and proper validated usage of all of the services, to the members of this Program Project Group in a way that ensures the rigor and reproducibility of all of the experiments. Investigators in the various Projects of this PPG will use Core A by first consulting with senior staff to define the goals for the experiments and prepare a protocol to achieve these goals. The staff of this core will then work with a representative from the project to execute the experiment, with the core staff supplying the hands-on skill during the demanding steps in animal surgery and handling, imaging and data collection to ensure uniformity of application of the method and the quality control needed to obtain and interpret the data. Without the services provided by Core A, our understanding of these processes would be derived from model systems and/or end-stage assays that only approximate the physiology of lung metastasis. In contrast, our approach is uniquely able to provide information about mechanism of in vivo behavior at the single cell level.