Advanced Imaging Core

项目摘要/摘要 高级成像核心为该计划项目组的每个项目提供人员配备， 评估和测试肿瘤转移机制所需的专业知识、设备和解释技能 肺部的次要部位。所提供的服务是此核心所独有的，因为它们是从 年内若干独立实验室在显微镜和活体成像方面取得的进展 爱因斯坦的格拉斯-理柏生物光子学中心。正如研究战略中详细描述的那样，这些 服务包括1)定制的多激光多光子显微镜；2)生命中的纵向成像 单细胞分辨率；3)活体肺和其他二次部位成像；4)活体细胞命运定位；5)大细胞 体积高分辨率活体内成像(LVHR-IVI)；6)茎干生物传感器储存库， 休眠、侵袭和缺氧；7)相关显微镜结合活体成像和 组织病理学(IVITA)；以及8)用于固定组织研究的定量数字病理学服务。总体而言， 使用先进的多光子显微镜；准备动物进行成像，包括麻醉和 外科手术；分析和解释收集的数据需要通过实践培养技能。 凭借敬业的专家工作人员，Core A将集中所需的设备和知识库 确保对本计划成员的所有服务进行优先访问和正确验证的使用 确保所有实验的严密性和可重复性。调查人员在 该PPG的各种项目将使用核心A，首先咨询高级员工，以确定 进行实验并准备一份协议来实现这些目标。然后，该核心的工作人员将使用 来自项目的代表执行实验，核心员工提供动手技能 在动物手术和处理、成像和数据收集等要求苛刻的步骤中，确保一致性 该方法的应用情况以及获取和解释数据所需的质量控制。如果没有 核心A提供的服务，我们对这些流程的理解将来自模型系统 和/或仅与肺转移的生理学相似的终末期分析。相比之下，我们的方法 是唯一能够在单细胞水平上提供体内行为机制的信息。

Project Summary/Abstract The Advanced Imaging Core provides to each of the projects of this Program Project Group the staffing, expertise, equipment, and interpretive skills needed to evaluate and test mechanisms of metastasis at the secondary site of the lung. The services provided are unique to this Core as they have been assembled from advances in microscopy and intravital imaging made by a number of independent laboratories within Einstein’s Gruss-Lipper Biophotonics center. As described in detail within the research strategy, these services consist of 1) A custom-built multi-laser multiphoton microscope; 2) Longitudinal intravital imaging at single cell resolution; 3) In vivo lung and other secondary site imaging; 4) In vivo cell fate mapping; 5) Large- Volume High-Resolution Intravital Imaging (LVHR-IVI); 6) A repository of biosensors for stemness, dormancy, invasiveness, and hypoxia; 7) Correlative microscopy combining intravital imaging with histopathology (IVITA); and 8) Quantitative digital pathology services for fixed tissue studies. In general, the use of advanced multiphoton microscopy; the preparation of animals for imaging including anesthesia and surgery; and the analysis and interpretation of the data collected require skill developed through practice. With dedicated expert staff members, Core A will centralize the equipment and knowledgebase needed to ensure prioritized access, and proper validated usage of all of the services, to the members of this Program Project Group in a way that ensures the rigor and reproducibility of all of the experiments. Investigators in the various Projects of this PPG will use Core A by first consulting with senior staff to define the goals for the experiments and prepare a protocol to achieve these goals. The staff of this core will then work with a representative from the project to execute the experiment, with the core staff supplying the hands-on skill during the demanding steps in animal surgery and handling, imaging and data collection to ensure uniformity of application of the method and the quality control needed to obtain and interpret the data. Without the services provided by Core A, our understanding of these processes would be derived from model systems and/or end-stage assays that only approximate the physiology of lung metastasis. In contrast, our approach is uniquely able to provide information about mechanism of in vivo behavior at the single cell level.